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- W2000550089 abstract "Nine-day chicken embryo neuroretinal cells transdifferentiate into both lens and pigment cells after 3-4 weeks when cultured in MEM medium containing 10% foetal calf serum at pH 7.4. At pH 6.8. the appearance of lens crystallins is retarded and cholineacetyltransferase (CAT) activity persists for longer, whereas at pH 8.0 crystallins appear earlier and CAT activity declines more rapidly. Cell survival and culture growth are about 10% lower at pH 6.8 than at pH 8.0. If the concentration of foetal calf serum (FCS) is increased from 10% to 25% (at pH 7.4), cell survival and growth are both promoted, crystallins appear slightly earlier and CAT activity declines more rapidly. Converse effects are observed with 5 % serum, accumulation of crystallins being greatly inhibited and CAT activity prolonged. Crystallin production in cultures with 10% or 25% chicken serum (CS) is much less extensive than in similar FCS cultures, but in cultures with 5 % CS, crystallins appear more rapidly, reaching higher levels than in 5 % FCS cultures. However, the pattern of CAT activity in response to different serum levels is similar for both CS and FCS. This might imply the presence of some factor(s) able to stimulate transdifferentiation in FCS, whereas CS can apparently inhibit this process." @default.
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- W2000550089 title "Alterations in pH and Serum Concentration have Contrasting Effects on Normal and Foreign Pathways of Differentiation in Cultures of Embryonic Chick Neuroretinal Cells" @default.
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- W2000550089 doi "https://doi.org/10.1111/j.1440-169x.1981.00613.x" @default.
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