Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000551115> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2000551115 endingPage "378a" @default.
- W2000551115 startingPage "378a" @default.
- W2000551115 abstract "TRPP3 channels or PKD2L1 channels, with high homology to another member of TRPP family, PKD2 (named after polycystic kidney disease), play potential roles in sour taste. TRPP3 channels are highly regulated by calcium, complicated with both calcium dependent activation and inactivation (Chen XZ, et al. 1999). However, the essential mechanisms of these calcium regulations are lacking (Li Q, et al., 2002). In this study, we took advantage of the unique off-response (Ioff) evoked by acid stimuli onto TRPP3 channels expressed in HEK 293 cells, to dissect the calcium dependent inactivation (CDI) from the complex calcium responses evidenced in oocyte preparations. By changing extracellular calcium concentrations from 0 to 110 mM, and intracellular buffers from EGTA to BAPTA, we proved that the decaying phase of Ioff is completely calcium dependent. We tested and then rejected the hypothesis that calmodulin mediates the CDI of TRPP3 channels, by overexpressing the mutant form of calmodulin. Further mutagenesis analyses revealed that CDI would be abolished when the interactions between intracellular calcium ions and the EF-hand of carboxyl termini of TRPP3 channels were perturbed. Our work not only provides mechanistic insights into CDI of Ioff, but also paves the way to the elucidation of calcium-dependent activation, thus the ultimate resolution of calcium-dependent regulations of TRPP3 channels." @default.
- W2000551115 created "2016-06-24" @default.
- W2000551115 creator A5035168940 @default.
- W2000551115 creator A5071165373 @default.
- W2000551115 creator A5089415584 @default.
- W2000551115 date "2013-01-01" @default.
- W2000551115 modified "2023-09-27" @default.
- W2000551115 title "Calcium-Dependent Regulations of TRPP3 Channels" @default.
- W2000551115 doi "https://doi.org/10.1016/j.bpj.2012.11.2106" @default.
- W2000551115 hasPublicationYear "2013" @default.
- W2000551115 type Work @default.
- W2000551115 sameAs 2000551115 @default.
- W2000551115 citedByCount "0" @default.
- W2000551115 crossrefType "journal-article" @default.
- W2000551115 hasAuthorship W2000551115A5035168940 @default.
- W2000551115 hasAuthorship W2000551115A5071165373 @default.
- W2000551115 hasAuthorship W2000551115A5089415584 @default.
- W2000551115 hasBestOaLocation W20005511151 @default.
- W2000551115 hasConcept C12554922 @default.
- W2000551115 hasConcept C155164980 @default.
- W2000551115 hasConcept C170493617 @default.
- W2000551115 hasConcept C174510640 @default.
- W2000551115 hasConcept C178790620 @default.
- W2000551115 hasConcept C181080969 @default.
- W2000551115 hasConcept C185592680 @default.
- W2000551115 hasConcept C18699975 @default.
- W2000551115 hasConcept C201571599 @default.
- W2000551115 hasConcept C2776610495 @default.
- W2000551115 hasConcept C2777658833 @default.
- W2000551115 hasConcept C2780019300 @default.
- W2000551115 hasConcept C28406088 @default.
- W2000551115 hasConcept C29688787 @default.
- W2000551115 hasConcept C30979828 @default.
- W2000551115 hasConcept C519063684 @default.
- W2000551115 hasConcept C55493867 @default.
- W2000551115 hasConcept C86803240 @default.
- W2000551115 hasConcept C95444343 @default.
- W2000551115 hasConceptScore W2000551115C12554922 @default.
- W2000551115 hasConceptScore W2000551115C155164980 @default.
- W2000551115 hasConceptScore W2000551115C170493617 @default.
- W2000551115 hasConceptScore W2000551115C174510640 @default.
- W2000551115 hasConceptScore W2000551115C178790620 @default.
- W2000551115 hasConceptScore W2000551115C181080969 @default.
- W2000551115 hasConceptScore W2000551115C185592680 @default.
- W2000551115 hasConceptScore W2000551115C18699975 @default.
- W2000551115 hasConceptScore W2000551115C201571599 @default.
- W2000551115 hasConceptScore W2000551115C2776610495 @default.
- W2000551115 hasConceptScore W2000551115C2777658833 @default.
- W2000551115 hasConceptScore W2000551115C2780019300 @default.
- W2000551115 hasConceptScore W2000551115C28406088 @default.
- W2000551115 hasConceptScore W2000551115C29688787 @default.
- W2000551115 hasConceptScore W2000551115C30979828 @default.
- W2000551115 hasConceptScore W2000551115C519063684 @default.
- W2000551115 hasConceptScore W2000551115C55493867 @default.
- W2000551115 hasConceptScore W2000551115C86803240 @default.
- W2000551115 hasConceptScore W2000551115C95444343 @default.
- W2000551115 hasIssue "2" @default.
- W2000551115 hasLocation W20005511151 @default.
- W2000551115 hasOpenAccess W2000551115 @default.
- W2000551115 hasPrimaryLocation W20005511151 @default.
- W2000551115 hasRelatedWork W159924709 @default.
- W2000551115 hasRelatedWork W2000551115 @default.
- W2000551115 hasRelatedWork W2036845897 @default.
- W2000551115 hasRelatedWork W2053909751 @default.
- W2000551115 hasRelatedWork W2055407120 @default.
- W2000551115 hasRelatedWork W2068423076 @default.
- W2000551115 hasRelatedWork W2071963132 @default.
- W2000551115 hasRelatedWork W2087201898 @default.
- W2000551115 hasRelatedWork W2110356796 @default.
- W2000551115 hasRelatedWork W878838041 @default.
- W2000551115 hasVolume "104" @default.
- W2000551115 isParatext "false" @default.
- W2000551115 isRetracted "false" @default.
- W2000551115 magId "2000551115" @default.
- W2000551115 workType "article" @default.