Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000558009> ?p ?o ?g. }
- W2000558009 endingPage "718" @default.
- W2000558009 startingPage "708" @default.
- W2000558009 abstract "Abstract Laboratory adaptive evolution of microorganisms offers the possibility of relating acquired mutations to increased fitness of the organism under the conditions used. By combining a fibrous‐bed bioreactor, we successfully developed a simple and valuable adaptive evolution strategy in repeated‐batch fermentation mode with high initial substrate concentration and evolved Clostridium tyrobutyricum mutant with significantly improved butyric acid volumetric productivity up to 2.25 g/(L h), which is the highest value in batch fermentation reported so far. Further experiments were conducted to pay attention to glucose transport system in consideration of the high glucose consumption rate resulted from evolution. Complete characterization and comparison of the glucose phosphoenolpyruvate (PEP)‐dependent phosphotransferase system (PTS) were carried out in the form of toluene‐treated cells and cell‐free extracts derived from both C. tyrobutyricum wide‐type and mutant, while an alternative glucose transport route that requires glucokinase was confirmed by the phenomena of resistance to the glucose analogue 2‐deoxyglucose and ATP‐dependent glucose phosphorylation. Our results suggest that C. tyrobutyricum mutant is defective in PTS activity and compensates for this defect with enhanced glucokinase activity, resulting in the efficient uptake and consumption of glucose during the whole metabolism. Biotechnol. Bioeng. 2012; 109:708–718. © 2011 Wiley Periodicals, Inc." @default.
- W2000558009 created "2016-06-24" @default.
- W2000558009 creator A5026954484 @default.
- W2000558009 creator A5028914473 @default.
- W2000558009 creator A5031719989 @default.
- W2000558009 creator A5061084605 @default.
- W2000558009 creator A5082349291 @default.
- W2000558009 date "2011-12-20" @default.
- W2000558009 modified "2023-10-18" @default.
- W2000558009 title "Adaptive evolution for fast growth on glucose and the effects on the regulation of glucose transport system in Clostridium tyrobutyricum" @default.
- W2000558009 cites W138805889 @default.
- W2000558009 cites W1596894355 @default.
- W2000558009 cites W1605823121 @default.
- W2000558009 cites W1755153389 @default.
- W2000558009 cites W1970172657 @default.
- W2000558009 cites W1973183517 @default.
- W2000558009 cites W1980740090 @default.
- W2000558009 cites W1992124657 @default.
- W2000558009 cites W2001764679 @default.
- W2000558009 cites W2005479418 @default.
- W2000558009 cites W2017477075 @default.
- W2000558009 cites W2017482443 @default.
- W2000558009 cites W2026361068 @default.
- W2000558009 cites W2026394130 @default.
- W2000558009 cites W2027701781 @default.
- W2000558009 cites W2031437537 @default.
- W2000558009 cites W2039240597 @default.
- W2000558009 cites W2041263034 @default.
- W2000558009 cites W2043218103 @default.
- W2000558009 cites W2044643353 @default.
- W2000558009 cites W2048112198 @default.
- W2000558009 cites W2050109294 @default.
- W2000558009 cites W2051207673 @default.
- W2000558009 cites W2051462457 @default.
- W2000558009 cites W2057209270 @default.
- W2000558009 cites W2057665989 @default.
- W2000558009 cites W2065916067 @default.
- W2000558009 cites W2068681532 @default.
- W2000558009 cites W2084529529 @default.
- W2000558009 cites W2087769036 @default.
- W2000558009 cites W2105842455 @default.
- W2000558009 cites W2122248052 @default.
- W2000558009 cites W2136107296 @default.
- W2000558009 cites W2136343510 @default.
- W2000558009 cites W2136699249 @default.
- W2000558009 cites W2138956739 @default.
- W2000558009 cites W2140653551 @default.
- W2000558009 cites W2148277388 @default.
- W2000558009 cites W2169758150 @default.
- W2000558009 cites W2216617737 @default.
- W2000558009 cites W2412052381 @default.
- W2000558009 doi "https://doi.org/10.1002/bit.23346" @default.
- W2000558009 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21956266" @default.
- W2000558009 hasPublicationYear "2011" @default.
- W2000558009 type Work @default.
- W2000558009 sameAs 2000558009 @default.
- W2000558009 citedByCount "32" @default.
- W2000558009 countsByYear W20005580092013 @default.
- W2000558009 countsByYear W20005580092014 @default.
- W2000558009 countsByYear W20005580092015 @default.
- W2000558009 countsByYear W20005580092016 @default.
- W2000558009 countsByYear W20005580092017 @default.
- W2000558009 countsByYear W20005580092018 @default.
- W2000558009 countsByYear W20005580092019 @default.
- W2000558009 countsByYear W20005580092020 @default.
- W2000558009 countsByYear W20005580092021 @default.
- W2000558009 crossrefType "journal-article" @default.
- W2000558009 hasAuthorship W2000558009A5026954484 @default.
- W2000558009 hasAuthorship W2000558009A5028914473 @default.
- W2000558009 hasAuthorship W2000558009A5031719989 @default.
- W2000558009 hasAuthorship W2000558009A5061084605 @default.
- W2000558009 hasAuthorship W2000558009A5082349291 @default.
- W2000558009 hasConcept C100544194 @default.
- W2000558009 hasConcept C104317684 @default.
- W2000558009 hasConcept C143065580 @default.
- W2000558009 hasConcept C143937172 @default.
- W2000558009 hasConcept C150903083 @default.
- W2000558009 hasConcept C161573976 @default.
- W2000558009 hasConcept C168170006 @default.
- W2000558009 hasConcept C17093226 @default.
- W2000558009 hasConcept C181199279 @default.
- W2000558009 hasConcept C185592680 @default.
- W2000558009 hasConcept C2779306644 @default.
- W2000558009 hasConcept C45656491 @default.
- W2000558009 hasConcept C55493867 @default.
- W2000558009 hasConcept C59822182 @default.
- W2000558009 hasConcept C86803240 @default.
- W2000558009 hasConcept C98335778 @default.
- W2000558009 hasConceptScore W2000558009C100544194 @default.
- W2000558009 hasConceptScore W2000558009C104317684 @default.
- W2000558009 hasConceptScore W2000558009C143065580 @default.
- W2000558009 hasConceptScore W2000558009C143937172 @default.
- W2000558009 hasConceptScore W2000558009C150903083 @default.
- W2000558009 hasConceptScore W2000558009C161573976 @default.
- W2000558009 hasConceptScore W2000558009C168170006 @default.
- W2000558009 hasConceptScore W2000558009C17093226 @default.
- W2000558009 hasConceptScore W2000558009C181199279 @default.
- W2000558009 hasConceptScore W2000558009C185592680 @default.