FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000561278> ?p ?o ?g. }

• W2000561278 endingPage "26" @default.
• W2000561278 startingPage "19" @default.
• W2000561278 abstract "There are now seven nucleoside/tide analogues, along with interferon-α, that are approved by the FDA for the management of chronic hepatitis B virus (HBV) infection, a disease affecting hundreds of millions of people worldwide. These medications, however, are limited in usefulness, and significant side effects and the emergence of viral escape mutants make the development of novel and updated therapeutics a pressing need in the treatment of HBV. With this in mind, a library containing 2000 compounds already known to be safe in both humans and mice with known mechanisms of action in mammalian cells were tested for the possibility of either antiviral activity against HBV or selective toxicity in HBV producing cell lines. A modified real-time immune-absorbance-polymerase chain reaction (IA-PCR) assay was developed for this screen, utilizing cells that produce and secrete intact HBV virions. In this procedure, viral particles are first captured by an anti-HBs antibody immobilized on a plate. The viral load is subsequently assessed by real-time PCR directly on captured particles. Using this assay, eight compounds were shown to consistently reduce the amount of secreted HBV viral particles in the culture medium under conditions that had no detectable impact on cell viability. Two compounds, proparacaine and chlorophyllide, were shown to reduce HBV levels 4- to 6-fold with an IC50 of 1 and 1.5 μM, respectively, and were selected for further study. The identification of these compounds as promising antiviral drug candidates against HBV, despite a lack of previous recognition of HBV antiviral activity, supports the validity and utility of testing known compounds for “off-pathogen target” activity against HBV, and also validates this IA-PCR assay as an important tool for the detection of anti-viral activity against enveloped viruses." @default.
• W2000561278 created "2016-06-24" @default.
• W2000561278 creator A5006071368 @default.
• W2000561278 creator A5012205087 @default.
• W2000561278 creator A5036469976 @default.
• W2000561278 creator A5041693287 @default.
• W2000561278 creator A5043567166 @default.
• W2000561278 creator A5045284378 @default.
• W2000561278 creator A5048193262 @default.
• W2000561278 creator A5052835805 @default.
• W2000561278 creator A5075977831 @default.
• W2000561278 date "2013-04-01" @default.
• W2000561278 modified "2023-09-26" @default.
• W2000561278 title "Screening and identification of compounds with antiviral activity against hepatitis B virus using a safe compound library and novel real-time immune-absorbance PCR-based high throughput system" @default.
• W2000561278 cites W1513593427 @default.
• W2000561278 cites W1880341895 @default.
• W2000561278 cites W1962682498 @default.
• W2000561278 cites W1979796678 @default.
• W2000561278 cites W1981844439 @default.
• W2000561278 cites W1984019342 @default.
• W2000561278 cites W1986423407 @default.
• W2000561278 cites W1991001144 @default.
• W2000561278 cites W1997965188 @default.
• W2000561278 cites W2016843393 @default.
• W2000561278 cites W2022515140 @default.
• W2000561278 cites W2031579836 @default.
• W2000561278 cites W2045745022 @default.
• W2000561278 cites W2048817520 @default.
• W2000561278 cites W2050456662 @default.
• W2000561278 cites W2058926495 @default.
• W2000561278 cites W2060710292 @default.
• W2000561278 cites W2061835342 @default.
• W2000561278 cites W2064622635 @default.
• W2000561278 cites W2067079120 @default.
• W2000561278 cites W2070339314 @default.
• W2000561278 cites W2090820796 @default.
• W2000561278 cites W2102130139 @default.
• W2000561278 cites W2108155748 @default.
• W2000561278 cites W2114688500 @default.
• W2000561278 cites W2121934317 @default.
• W2000561278 cites W2128014215 @default.
• W2000561278 cites W2137218964 @default.
• W2000561278 cites W2143233396 @default.
• W2000561278 cites W2164665317 @default.
• W2000561278 cites W4233571696 @default.
• W2000561278 doi "https://doi.org/10.1016/j.antiviral.2013.02.001" @default.
• W2000561278 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3628437" @default.
• W2000561278 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23415884" @default.
• W2000561278 hasPublicationYear "2013" @default.
• W2000561278 type Work @default.
• W2000561278 sameAs 2000561278 @default.
• W2000561278 citedByCount "14" @default.
• W2000561278 countsByYear W20005612782014 @default.
• W2000561278 countsByYear W20005612782015 @default.
• W2000561278 countsByYear W20005612782016 @default.
• W2000561278 countsByYear W20005612782018 @default.
• W2000561278 countsByYear W20005612782019 @default.
• W2000561278 countsByYear W20005612782020 @default.
• W2000561278 countsByYear W20005612782021 @default.
• W2000561278 countsByYear W20005612782022 @default.
• W2000561278 crossrefType "journal-article" @default.
• W2000561278 hasAuthorship W2000561278A5006071368 @default.
• W2000561278 hasAuthorship W2000561278A5012205087 @default.
• W2000561278 hasAuthorship W2000561278A5036469976 @default.
• W2000561278 hasAuthorship W2000561278A5041693287 @default.
• W2000561278 hasAuthorship W2000561278A5043567166 @default.
• W2000561278 hasAuthorship W2000561278A5045284378 @default.
• W2000561278 hasAuthorship W2000561278A5048193262 @default.
• W2000561278 hasAuthorship W2000561278A5052835805 @default.
• W2000561278 hasAuthorship W2000561278A5075977831 @default.
• W2000561278 hasBestOaLocation W20005612782 @default.
• W2000561278 hasConcept C142462285 @default.
• W2000561278 hasConcept C159047783 @default.
• W2000561278 hasConcept C185592680 @default.
• W2000561278 hasConcept C203014093 @default.
• W2000561278 hasConcept C2522874641 @default.
• W2000561278 hasConcept C2776178377 @default.
• W2000561278 hasConcept C2776543447 @default.
• W2000561278 hasConcept C2777333352 @default.
• W2000561278 hasConcept C2777382497 @default.
• W2000561278 hasConcept C2779756493 @default.
• W2000561278 hasConcept C2780593183 @default.
• W2000561278 hasConcept C54355233 @default.
• W2000561278 hasConcept C55493867 @default.
• W2000561278 hasConcept C81885089 @default.
• W2000561278 hasConcept C86803240 @default.
• W2000561278 hasConcept C8891405 @default.
• W2000561278 hasConceptScore W2000561278C142462285 @default.
• W2000561278 hasConceptScore W2000561278C159047783 @default.
• W2000561278 hasConceptScore W2000561278C185592680 @default.
• W2000561278 hasConceptScore W2000561278C203014093 @default.
• W2000561278 hasConceptScore W2000561278C2522874641 @default.
• W2000561278 hasConceptScore W2000561278C2776178377 @default.
• W2000561278 hasConceptScore W2000561278C2776543447 @default.
• W2000561278 hasConceptScore W2000561278C2777333352 @default.
• W2000561278 hasConceptScore W2000561278C2777382497 @default.
• W2000561278 hasConceptScore W2000561278C2779756493 @default.
• W2000561278 hasConceptScore W2000561278C2780593183 @default.

• next