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• W2000568248 abstract "The protective effects of adenovirus-mediated glia cell line-derived neurotrophic factor (GDNF) gene transaction was investigated on cultured motoneurons. First, the dose- and time-response relationship of glutamate neurotoxicity was determined on spinal motoneuron cultures. Then, the effect of the gdnf recombinant adenovirus (AdCMVgdnf) was tested in this cellular model. AdCMVgdnf at 20 MOI (multiplicity of infection) was found to significantly reduce the cell loss of motoneurons, as compared to AdCMVgdnf at 20 MOI, the recombinant adenovirus containing the marker gene lacZ. Furthermore, the adenovirus was proved to mediate erogenous gene expression using X-Gal staining and a semi-quantitative RT-PCR method. These results suggested a therapeutic potential of adenovirus vector-mediated gdnf gene therapy in human motoneuron diseases." @default.
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• W2000568248 date "2001-10-01" @default.
• W2000568248 modified "2023-09-26" @default.
• W2000568248 title "Adenovirus-mediated GDNF protects cultured motoneurons from glutamate injury" @default.
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• W2000568248 doi "https://doi.org/10.1097/00001756-200110080-00018" @default.
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