FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000569488> ?p ?o ?g. }

• W2000569488 endingPage "3095" @default.
• W2000569488 startingPage "3085" @default.
• W2000569488 abstract "Calcineurin is a Ca (2+)/calmodulin-activated Ser/Thr phosphatase important in cellular actions resulting in memory formation, cardiac hypertrophy, and T-cell activation. This enzyme is subject to oxidative inactivation by superoxide at low micromolar concentrations and by H 2O 2 at low millimolar concentrations. On the basis of the hypothesis that oxidation of Met residues in calmodulin-binding domains inhibits binding to calmodulin, purified calcineurin was used to study the susceptibility of Met residues to oxidation by H 2O 2. The rate for oxidation of Met 406 in the calmodulin-binding domain was determined to be 4.4 x 10 (-3) M (-1) s (-1), indicating a high susceptibility to oxidation. Functional repercussions of Met 406 oxidation were evaluated using native enzyme and a calcineurin mutant in which Met 406 was exchanged for Leu. Measurement of fluorescent calmodulin binding demonstrated that oxidation of Met 406 results in a 3.3-fold decrease in the affinity of calmodulin for calcineurin. Calcineurin activation exhibited a loss in cooperativity with respect to calmodulin following Met 406 oxidation as shown by a reduction in the Hill slope from 1.88 to 0.86. Maximum phosphatase activity was unaffected by Met oxidation. Changes in the calcineurin-calmodulin interaction were accompanied by a 40% loss in the ability of calmodulin to stimulate binding of immunophilin/immunosuppressant to calcineurin. All effects on calmodulin binding to the native enzyme by the treatment with H 2O 2 could be reversed by treating the enzyme with methionine sulfoxide reductase. These results indicate that the calmodulin-binding domain of calcineurin is susceptible to oxidation at Met 406 and that oxidation disrupts calmodulin binding and enzyme activation. Oxidation-dependent decreases in the affinity of calmodulin for calcineurin can potentially modulate calmodulin-dependent signaling and calmodulin distribution." @default.
• W2000569488 created "2016-06-24" @default.
• W2000569488 creator A5007165478 @default.
• W2000569488 creator A5049983845 @default.
• W2000569488 date "2008-02-15" @default.
• W2000569488 modified "2023-09-27" @default.
• W2000569488 title "Methionine Oxidation in the Calmodulin-Binding Domain of Calcineurin Disrupts Calmodulin Binding and Calcineurin Activation" @default.
• W2000569488 cites W1492276448 @default.
• W2000569488 cites W1545733166 @default.
• W2000569488 cites W1767701883 @default.
• W2000569488 cites W1967641078 @default.
• W2000569488 cites W1969348776 @default.
• W2000569488 cites W1972143467 @default.
• W2000569488 cites W1987159249 @default.
• W2000569488 cites W1995023903 @default.
• W2000569488 cites W1998797784 @default.
• W2000569488 cites W1999229913 @default.
• W2000569488 cites W1999921360 @default.
• W2000569488 cites W2001361000 @default.
• W2000569488 cites W2008166558 @default.
• W2000569488 cites W2011836829 @default.
• W2000569488 cites W2012206465 @default.
• W2000569488 cites W2018101787 @default.
• W2000569488 cites W2020562971 @default.
• W2000569488 cites W2021300391 @default.
• W2000569488 cites W2022025232 @default.
• W2000569488 cites W2023842903 @default.
• W2000569488 cites W2025679060 @default.
• W2000569488 cites W2026258231 @default.
• W2000569488 cites W2026388670 @default.
• W2000569488 cites W2033212918 @default.
• W2000569488 cites W2033938885 @default.
• W2000569488 cites W2036272929 @default.
• W2000569488 cites W2037738972 @default.
• W2000569488 cites W2039042657 @default.
• W2000569488 cites W2040996409 @default.
• W2000569488 cites W2041040796 @default.
• W2000569488 cites W2049894741 @default.
• W2000569488 cites W2054994243 @default.
• W2000569488 cites W2061394468 @default.
• W2000569488 cites W2068265376 @default.
• W2000569488 cites W2072678654 @default.
• W2000569488 cites W2074593407 @default.
• W2000569488 cites W2080025627 @default.
• W2000569488 cites W2086141071 @default.
• W2000569488 cites W2107501339 @default.
• W2000569488 cites W2115592403 @default.
• W2000569488 cites W2116153674 @default.
• W2000569488 cites W2118771925 @default.
• W2000569488 cites W2141589798 @default.
• W2000569488 cites W2159976847 @default.
• W2000569488 cites W2169569915 @default.
• W2000569488 cites W2485101139 @default.
• W2000569488 doi "https://doi.org/10.1021/bi702044x" @default.
• W2000569488 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4167793" @default.
• W2000569488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18275158" @default.
• W2000569488 hasPublicationYear "2008" @default.
• W2000569488 type Work @default.
• W2000569488 sameAs 2000569488 @default.
• W2000569488 citedByCount "55" @default.
• W2000569488 countsByYear W20005694882012 @default.
• W2000569488 countsByYear W20005694882013 @default.
• W2000569488 countsByYear W20005694882014 @default.
• W2000569488 countsByYear W20005694882015 @default.
• W2000569488 countsByYear W20005694882016 @default.
• W2000569488 countsByYear W20005694882017 @default.
• W2000569488 countsByYear W20005694882018 @default.
• W2000569488 countsByYear W20005694882019 @default.
• W2000569488 countsByYear W20005694882020 @default.
• W2000569488 countsByYear W20005694882021 @default.
• W2000569488 crossrefType "journal-article" @default.
• W2000569488 hasAuthorship W2000569488A5007165478 @default.
• W2000569488 hasAuthorship W2000569488A5049983845 @default.
• W2000569488 hasBestOaLocation W20005694882 @default.
• W2000569488 hasConcept C126322002 @default.
• W2000569488 hasConcept C128057223 @default.
• W2000569488 hasConcept C178666793 @default.
• W2000569488 hasConcept C181199279 @default.
• W2000569488 hasConcept C185592680 @default.
• W2000569488 hasConcept C2911091166 @default.
• W2000569488 hasConcept C29688787 @default.
• W2000569488 hasConcept C55493867 @default.
• W2000569488 hasConcept C71924100 @default.
• W2000569488 hasConceptScore W2000569488C126322002 @default.
• W2000569488 hasConceptScore W2000569488C128057223 @default.
• W2000569488 hasConceptScore W2000569488C178666793 @default.
• W2000569488 hasConceptScore W2000569488C181199279 @default.
• W2000569488 hasConceptScore W2000569488C185592680 @default.
• W2000569488 hasConceptScore W2000569488C2911091166 @default.
• W2000569488 hasConceptScore W2000569488C29688787 @default.
• W2000569488 hasConceptScore W2000569488C55493867 @default.
• W2000569488 hasConceptScore W2000569488C71924100 @default.
• W2000569488 hasIssue "10" @default.
• W2000569488 hasLocation W20005694881 @default.
• W2000569488 hasLocation W20005694882 @default.
• W2000569488 hasLocation W20005694883 @default.
• W2000569488 hasLocation W20005694884 @default.
• W2000569488 hasOpenAccess W2000569488 @default.

• next