FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000578504> ?p ?o ?g. }

• W2000578504 endingPage "78" @default.
• W2000578504 startingPage "71" @default.
• W2000578504 abstract "The mitochondrion plays a crucial role in the process of apoptosis and has thus become one of the targets for the search for potential chemotherapeutic agents. Betulinic acid [3β-hydroxy-lup-20(19)lupaen-28-carbonic acid], a lupane-type triterpene which is abundant in many plant species, has been shown to exert a direct effect on the mitochondria and subsequent apoptosis in melanoma cells. Chemical synthesis and modification of betulinic acid are being explored to develop more potent derivatives. We present here the apoptotic activity of several natural derivatives of betulinic acid which were isolated from the roots of a Chinese medicinal herb, Pulsatilla chinensis (Bge) Regel [Ye, W., Ji, N.N., Zhao, S.X., Liu, J.H., Ye, T., McKervey, M.A., Stevenson, P., 1996. Triterpenoids from Pulsatilla chinensis. Phytochemistry 42, 799–802]. Of the five compounds tested, 3-oxo-23-hydroxybetulinic acid was the most cytotoxic on murine melanoma B16 cells (IC50=22.5 μg/ml), followed by 23-hydroxybetulinic acid and betulinic acid (IC50=32 and 76 μg/ml, respectively), with lupeol and betulin exhibiting the weakest cytotoxicity (IC50≥100 μg/ml). Exposure of B16 cells to betulinic acid, 23-hydroxybetulinic acid and 3-oxo-23-hydroxybetulinic acid caused a rapid increase in reactive oxidative species production and a concomitant dissipation of mitochondrial membrane potential in a dose- and time-dependent manner, which resulted in cell apoptosis, as demonstrated by fluorescence microscopy, gel electrophoresis and flow-cytometric analysis. Cell cycle analysis further demonstrated that both 3-oxo-23-hydroxybetulinic acid and 23-hydroxybetulinic acid dramatically increased DNA fragmentation at the expense of G1 cells at doses as low as 12.5 and 25 μg/ml, respectively, thereby showing their potent apoptotic properties. Our results showed that hydroxylation at the C3 position of betulinic acid is likely to enhance the apoptotic activity of betulinic acid derivatives (23-hydroxybetulinic acid and 3-oxo-23-hydroxybetulinic acid) on murine melanoma B16 cells." @default.
• W2000578504 created "2016-06-24" @default.
• W2000578504 creator A5038830876 @default.
• W2000578504 creator A5047635178 @default.
• W2000578504 creator A5074832892 @default.
• W2000578504 creator A5082689762 @default.
• W2000578504 creator A5083448314 @default.
• W2000578504 creator A5088403366 @default.
• W2000578504 date "2004-09-01" @default.
• W2000578504 modified "2023-10-15" @default.
• W2000578504 title "Apoptotic activity of betulinic acid derivatives on murine melanoma B16 cell line" @default.
• W2000578504 cites W1975496726 @default.
• W2000578504 cites W1980169213 @default.
• W2000578504 cites W1993980819 @default.
• W2000578504 cites W1998175560 @default.
• W2000578504 cites W2000446104 @default.
• W2000578504 cites W2007313590 @default.
• W2000578504 cites W2014023055 @default.
• W2000578504 cites W2022573986 @default.
• W2000578504 cites W2039991473 @default.
• W2000578504 cites W2043316693 @default.
• W2000578504 cites W2052853635 @default.
• W2000578504 cites W2057045965 @default.
• W2000578504 cites W2057361927 @default.
• W2000578504 cites W2069051806 @default.
• W2000578504 cites W2074215098 @default.
• W2000578504 cites W2088512173 @default.
• W2000578504 cites W2093290099 @default.
• W2000578504 cites W2095193361 @default.
• W2000578504 cites W2102732416 @default.
• W2000578504 cites W2128697833 @default.
• W2000578504 cites W2135729408 @default.
• W2000578504 cites W2136966358 @default.
• W2000578504 cites W2155898968 @default.
• W2000578504 cites W2168455946 @default.
• W2000578504 doi "https://doi.org/10.1016/j.ejphar.2004.07.103" @default.
• W2000578504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15363977" @default.
• W2000578504 hasPublicationYear "2004" @default.
• W2000578504 type Work @default.
• W2000578504 sameAs 2000578504 @default.
• W2000578504 citedByCount "94" @default.
• W2000578504 countsByYear W20005785042012 @default.
• W2000578504 countsByYear W20005785042013 @default.
• W2000578504 countsByYear W20005785042014 @default.
• W2000578504 countsByYear W20005785042015 @default.
• W2000578504 countsByYear W20005785042016 @default.
• W2000578504 countsByYear W20005785042018 @default.
• W2000578504 countsByYear W20005785042019 @default.
• W2000578504 countsByYear W20005785042020 @default.
• W2000578504 countsByYear W20005785042021 @default.
• W2000578504 countsByYear W20005785042022 @default.
• W2000578504 countsByYear W20005785042023 @default.
• W2000578504 crossrefType "journal-article" @default.
• W2000578504 hasAuthorship W2000578504A5038830876 @default.
• W2000578504 hasAuthorship W2000578504A5047635178 @default.
• W2000578504 hasAuthorship W2000578504A5074832892 @default.
• W2000578504 hasAuthorship W2000578504A5082689762 @default.
• W2000578504 hasAuthorship W2000578504A5083448314 @default.
• W2000578504 hasAuthorship W2000578504A5088403366 @default.
• W2000578504 hasConcept C109316439 @default.
• W2000578504 hasConcept C185592680 @default.
• W2000578504 hasConcept C190283241 @default.
• W2000578504 hasConcept C202751555 @default.
• W2000578504 hasConcept C2777829905 @default.
• W2000578504 hasConcept C2778509883 @default.
• W2000578504 hasConcept C2779782153 @default.
• W2000578504 hasConcept C54355233 @default.
• W2000578504 hasConcept C55493867 @default.
• W2000578504 hasConcept C556039675 @default.
• W2000578504 hasConcept C71924100 @default.
• W2000578504 hasConcept C86803240 @default.
• W2000578504 hasConcept C98274493 @default.
• W2000578504 hasConceptScore W2000578504C109316439 @default.
• W2000578504 hasConceptScore W2000578504C185592680 @default.
• W2000578504 hasConceptScore W2000578504C190283241 @default.
• W2000578504 hasConceptScore W2000578504C202751555 @default.
• W2000578504 hasConceptScore W2000578504C2777829905 @default.
• W2000578504 hasConceptScore W2000578504C2778509883 @default.
• W2000578504 hasConceptScore W2000578504C2779782153 @default.
• W2000578504 hasConceptScore W2000578504C54355233 @default.
• W2000578504 hasConceptScore W2000578504C55493867 @default.
• W2000578504 hasConceptScore W2000578504C556039675 @default.
• W2000578504 hasConceptScore W2000578504C71924100 @default.
• W2000578504 hasConceptScore W2000578504C86803240 @default.
• W2000578504 hasConceptScore W2000578504C98274493 @default.
• W2000578504 hasIssue "1-3" @default.
• W2000578504 hasLocation W20005785041 @default.
• W2000578504 hasLocation W20005785042 @default.
• W2000578504 hasOpenAccess W2000578504 @default.
• W2000578504 hasPrimaryLocation W20005785041 @default.
• W2000578504 hasRelatedWork W1828389963 @default.
• W2000578504 hasRelatedWork W1979942106 @default.
• W2000578504 hasRelatedWork W2072347882 @default.
• W2000578504 hasRelatedWork W2335039477 @default.
• W2000578504 hasRelatedWork W2339045766 @default.
• W2000578504 hasRelatedWork W2404436289 @default.
• W2000578504 hasRelatedWork W2418247038 @default.
• W2000578504 hasRelatedWork W2804660990 @default.

• next