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• W2000593033 abstract "The mechanism of Fas antigen-induced hepatocyte apoptosis was investigated. Using a monoclonal antibody directed against the Fas antigen, apoptosis was induced in freshly isolated murine hepatocytes within 90 minutes of antibody addition as assessed by plasma membrane bleb formation, chromatin condensation, and DNA fragmentation. Pretreatment of the cells with the caspase inhibitors, N-acetyl-Asp-Glu-Val-Asp aldehyde (Ac-DEVD-CHO), benzyloxycarbonyl-Val-Ala-dl -Asp-fluoromethylketone (Z-VAD-FMK), or Z-Asp-2,6-dichlorobenzoyloxymethylketone inhibited anti-Fas-mediated apoptosis. Likewise, the serine protease inhibitors, N-tosyl-L -phenyl chloromethyl ketone (TPCK) and 3,4-dichloroisocoumarin (DCI), prevented apoptosis, whereas N-tosyl-L -lysine chloromethyl ketone (TLCK), Ac-Leu-Leu-L -norleucinal, Ac-Leu-Leu-L -methional, and trans-epoxysuccinyl-L -leucylamido-(4-guanidino)butane were without effect. Examination of CED-3/caspase-3-related caspases revealed that pro-caspases-3 (CPP32) and -7 (Mch-3α) were rapidly processed after Fas antigen stimulation. Caspase-7 was further cleaved to form the catalytically active subunits. In contrast, the p17 subunit of caspase-3 was not detected, indicating slow formation or rapid degradation. The activation of CED-3-related caspases was further confirmed by an increase in the rate of Z-DEVD-7-amino-4-trifluoromethylcoumarin (Z-DEVD-AFC) hydrolysis that was sensitive to Ac-DEVD-CHO and was inhibited by pretreatment of the cells with TPCK but not by DCI. In contrast, no increase in the rates of hydrolysis of Z-YVAD-AFC, a substrate for caspase-1, was detected. Investigation of the in situ proteolytic cleavage of the CED-3 related caspases substrate, poly(ADP-ribose) polymerase, revealed that this protein was not degraded in hepatocytes undergoing Fas-mediated apoptosis. Taken together, our results show that processing of caspases, in particular, caspases-7 and -3, occurs during Fas-induced apoptosis of mouse hepatocytes and suggest a role of these proteases as well as serine protease(s) in the apoptotic response." @default.
• W2000593033 created "2016-06-24" @default.
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• W2000593033 date "1998-06-01" @default.
• W2000593033 modified "2023-10-11" @default.
• W2000593033 title "Fas-mediated apoptosis in mouse hepatocytes involves the processing and activation of caspases" @default.
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• W2000593033 cites W1973243271 @default.
• W2000593033 cites W1976692354 @default.
• W2000593033 cites W1981106016 @default.
• W2000593033 cites W1981694464 @default.
• W2000593033 cites W1984696190 @default.
• W2000593033 cites W1986308149 @default.
• W2000593033 cites W1990917512 @default.
• W2000593033 cites W1991196959 @default.
• W2000593033 cites W1991501592 @default.
• W2000593033 cites W1993177726 @default.
• W2000593033 cites W1996180957 @default.
• W2000593033 cites W2002503549 @default.
• W2000593033 cites W2005825891 @default.
• W2000593033 cites W2007689766 @default.
• W2000593033 cites W2008261126 @default.
• W2000593033 cites W2010298858 @default.
• W2000593033 cites W2015608566 @default.
• W2000593033 cites W2016824586 @default.
• W2000593033 cites W2018949373 @default.
• W2000593033 cites W2019450833 @default.
• W2000593033 cites W2020247930 @default.
• W2000593033 cites W2021126994 @default.
• W2000593033 cites W2024708288 @default.
• W2000593033 cites W2030892254 @default.
• W2000593033 cites W2032417513 @default.
• W2000593033 cites W2037804577 @default.
• W2000593033 cites W2039355845 @default.
• W2000593033 cites W2047168565 @default.
• W2000593033 cites W2048620835 @default.
• W2000593033 cites W2049083444 @default.
• W2000593033 cites W2049110705 @default.
• W2000593033 cites W2052691929 @default.
• W2000593033 cites W2052853635 @default.
• W2000593033 cites W2057739013 @default.
• W2000593033 cites W2058970299 @default.
• W2000593033 cites W2061368292 @default.
• W2000593033 cites W2066096456 @default.
• W2000593033 cites W2067534266 @default.
• W2000593033 cites W2072502723 @default.
• W2000593033 cites W2074562878 @default.
• W2000593033 cites W2075177095 @default.
• W2000593033 cites W2078000179 @default.
• W2000593033 cites W2081145901 @default.
• W2000593033 cites W2083560313 @default.
• W2000593033 cites W2094334993 @default.
• W2000593033 cites W2094758923 @default.
• W2000593033 cites W2104543679 @default.
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• W2000593033 cites W2156552404 @default.
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• W2000593033 cites W2160141634 @default.
• W2000593033 cites W2161261662 @default.
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• W2000593033 doi "https://doi.org/10.1002/hep.510270624" @default.
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