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• W2000596036 abstract "The characterization of protein-protein interactions provides the foundation for further studies concerning protein complex function and regulation. Since the advent of the yeast two-hybrid assay, many additional genetic systems based upon the principle of protein fragment complementation have been designed. One such system, the split-ubiquitin membrane yeast two-hybrid system (MbYTH), is able to analyze the interaction status between two integral membrane proteins. This ability of the MbYTH system augments genetic analysis of protein interactions by covering for the inherent limitation of the yeast two-hybrid system when studying membrane protein interactions. Herein, we provide a description of the MbYTH method and detailed protocols in order to monitor protein interactions and discover novel interacting partners using the MbYTH system." @default.
• W2000596036 created "2016-06-24" @default.
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• W2000596036 date "2004-04-01" @default.
• W2000596036 modified "2023-10-16" @default.
• W2000596036 title "Application of the split-ubiquitin membrane yeast two-hybrid system to investigate membrane protein interactions" @default.
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• W2000596036 doi "https://doi.org/10.1016/j.ymeth.2003.10.010" @default.
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