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- W2000606178 abstract "A simple and versatile technique of horizontal slab gel electrofocusing is described which is based on a discontinuity of the gel volume along the pH axis achieved by overlaying ampholyte containing gel strips, of a defined volume and shape, at various locations on a base gel during the prerun of isoelectric focusing. In the electric field the overlaid gel strip delivers ampholytes of a distinct pI interval into the strip-free part of the base gel and removes from the latter some ampholytes present in the system. Flattening of the pH gradient in the strip-free part of the gel is the consequence. The technique is especially useful for thin gels and can be performed with any commercially available horizontal slab apparatus." @default.
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- W2000606178 date "1980-04-01" @default.
- W2000606178 modified "2023-09-27" @default.
- W2000606178 title "Flattening, shifting and comparing pH gradients in slab gel electrofocusing by local increases of gel volume" @default.
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- W2000606178 doi "https://doi.org/10.1002/elps.1150010110" @default.
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