FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000606346> ?p ?o ?g. }

• W2000606346 endingPage "373" @default.
• W2000606346 startingPage "361" @default.
• W2000606346 abstract "The Candida species are the most common fungal pathogens of systemic candidiasis. The diagnosis of invasive candidiasis remains a laboratory and clinical challenge. Thus, development of diagnostic assays to detect systemic candidiasis and to identify Candida virulence factors and associated pathogenesis through immunohistochemistry using specific monoclonals and polyclonals will be useful. Inbred Balb/c mice were immunized with C. albicans antigens, and blood was checked for the presence of reactive antibodies using ELISA. Fusion was performed using the harvested spleen cells and NS1 myeloma cells, and the clones were screened for the presence of antibody producing hybrid cells by dot-blot. Western blot analysis showed that the L2D10 monoclonal antibody was reactive against the antigens with molecular weight of 20 kDa. Experimental systemic candidiasis in mice was induced through intravenous injection of C. albicans and all the vital organs were collected for immunohistochemistry study. The monoclonal antibody reacted to surface epitopes on the yeast cells, germ tubes, and hyphae, and to immune complexes. It was used with the polyclonal antibody in a sandwich ELISA for the detection of circulating antigens in experimental candiadiasis in mice. Antibody levels were also determined using the ELISA method, and the antibody levels of C. albicans infected mice were increased compared with uninfected animals. The monoclonal antibody was used in immunoperoxidase and immunofluorescence techniques for the detection of fungal infection in tissue sections and was found to be more sensitive than conventional periodic acid Schiff or silver staining techniques. This monoclonal antibody may serve as potential primary capture antibodies for the development of a rapid diagnostic test for human systemic fungal infection." @default.
• W2000606346 created "2016-06-24" @default.
• W2000606346 creator A5002904533 @default.
• W2000606346 creator A5005862443 @default.
• W2000606346 creator A5037288894 @default.
• W2000606346 date "2008-10-01" @default.
• W2000606346 modified "2023-10-14" @default.
• W2000606346 title "Potential Use of a Monoclonal Antibody for the Detection of Candida Antigens in an Experimental Systemic Candidiasis Model" @default.
• W2000606346 cites W135858125 @default.
• W2000606346 cites W1522901991 @default.
• W2000606346 cites W1545570079 @default.
• W2000606346 cites W187108214 @default.
• W2000606346 cites W1917430189 @default.
• W2000606346 cites W1964300113 @default.
• W2000606346 cites W1964574920 @default.
• W2000606346 cites W1968911442 @default.
• W2000606346 cites W1991052155 @default.
• W2000606346 cites W1994038422 @default.
• W2000606346 cites W1994776671 @default.
• W2000606346 cites W1999218933 @default.
• W2000606346 cites W2005255684 @default.
• W2000606346 cites W2006980380 @default.
• W2000606346 cites W2008842477 @default.
• W2000606346 cites W2011699699 @default.
• W2000606346 cites W2020011206 @default.
• W2000606346 cites W2030522563 @default.
• W2000606346 cites W2032053330 @default.
• W2000606346 cites W2034524375 @default.
• W2000606346 cites W2036775339 @default.
• W2000606346 cites W2038206713 @default.
• W2000606346 cites W2043839530 @default.
• W2000606346 cites W2047659549 @default.
• W2000606346 cites W2053261584 @default.
• W2000606346 cites W2082396982 @default.
• W2000606346 cites W2087360209 @default.
• W2000606346 cites W2096913907 @default.
• W2000606346 cites W2100837269 @default.
• W2000606346 cites W2101108802 @default.
• W2000606346 cites W2114164442 @default.
• W2000606346 cites W2116871349 @default.
• W2000606346 cites W2117464846 @default.
• W2000606346 cites W2119384644 @default.
• W2000606346 cites W2121293410 @default.
• W2000606346 cites W2126476096 @default.
• W2000606346 cites W2133036434 @default.
• W2000606346 cites W2138563099 @default.
• W2000606346 cites W2144329905 @default.
• W2000606346 cites W2145450842 @default.
• W2000606346 cites W2149583552 @default.
• W2000606346 cites W2150616093 @default.
• W2000606346 cites W2152727338 @default.
• W2000606346 cites W2153104382 @default.
• W2000606346 cites W2164084399 @default.
• W2000606346 cites W2165208244 @default.
• W2000606346 cites W2168271732 @default.
• W2000606346 cites W2324205606 @default.
• W2000606346 cites W2096529182 @default.
• W2000606346 doi "https://doi.org/10.1089/hyb.2008.0021" @default.
• W2000606346 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18823263" @default.
• W2000606346 hasPublicationYear "2008" @default.
• W2000606346 type Work @default.
• W2000606346 sameAs 2000606346 @default.
• W2000606346 citedByCount "4" @default.
• W2000606346 crossrefType "journal-article" @default.
• W2000606346 hasAuthorship W2000606346A5002904533 @default.
• W2000606346 hasAuthorship W2000606346A5005862443 @default.
• W2000606346 hasAuthorship W2000606346A5037288894 @default.
• W2000606346 hasConcept C104317684 @default.
• W2000606346 hasConcept C128102431 @default.
• W2000606346 hasConcept C145081991 @default.
• W2000606346 hasConcept C147483822 @default.
• W2000606346 hasConcept C153911025 @default.
• W2000606346 hasConcept C159047783 @default.
• W2000606346 hasConcept C159654299 @default.
• W2000606346 hasConcept C195616568 @default.
• W2000606346 hasConcept C203014093 @default.
• W2000606346 hasConcept C204232928 @default.
• W2000606346 hasConcept C2776415932 @default.
• W2000606346 hasConcept C2778236600 @default.
• W2000606346 hasConcept C2779959462 @default.
• W2000606346 hasConcept C2780917455 @default.
• W2000606346 hasConcept C542903549 @default.
• W2000606346 hasConcept C55493867 @default.
• W2000606346 hasConcept C86803240 @default.
• W2000606346 hasConcept C89423630 @default.
• W2000606346 hasConcept C956191 @default.
• W2000606346 hasConceptScore W2000606346C104317684 @default.
• W2000606346 hasConceptScore W2000606346C128102431 @default.
• W2000606346 hasConceptScore W2000606346C145081991 @default.
• W2000606346 hasConceptScore W2000606346C147483822 @default.
• W2000606346 hasConceptScore W2000606346C153911025 @default.
• W2000606346 hasConceptScore W2000606346C159047783 @default.
• W2000606346 hasConceptScore W2000606346C159654299 @default.
• W2000606346 hasConceptScore W2000606346C195616568 @default.
• W2000606346 hasConceptScore W2000606346C203014093 @default.
• W2000606346 hasConceptScore W2000606346C204232928 @default.
• W2000606346 hasConceptScore W2000606346C2776415932 @default.
• W2000606346 hasConceptScore W2000606346C2778236600 @default.

• next