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• W2000606502 abstract "Rapid, sensitive, and sequence-specific DNA detection can be achieved in one step using an engineered intrasterically regulated enzyme. The semi-synthetic inhibitor-DNA-enzyme (IDE) construct (left) rests in the inactive state but upon exposure to a complementary DNA sequence undergoes a DNA hybridization-triggered allosteric enzyme activation (right). The ensuing rapid substrate turnover provides the built-in signal amplification mechanism for detecting approximately 10 fmol DNA in less than 3 min under physiological conditions." @default.
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• W2000606502 date "2002-12-17" @default.
• W2000606502 modified "2023-10-16" @default.
• W2000606502 title "DNA Detection and Signal Amplification via an Engineered Allosteric Enzyme" @default.
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• W2000606502 doi "https://doi.org/10.1021/ja027885u" @default.
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