FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000615699> ?p ?o ?g. }

• W2000615699 endingPage "947" @default.
• W2000615699 startingPage "938" @default.
• W2000615699 abstract "Severe acute respiratory syndrome (SARS) is an emerging infectious disease associated with a novel coronavirus and causing worldwide outbreaks. SARS coronavirus (SARS-CoV) is an enveloped RNA virus, which contains several structural proteins. Among these proteins, spike (S) protein is responsible for binding to specific cellular receptors and is a major antigenic determinant, which induces neutralizing antibody. In order to analyze the antigenicity and receptor-binding ability of SARS-CoV S protein, we expressed the S protein in Escherichia coli using a pET expression vector. After the isopropyl-beta-D-thiogalactoside induction, S protein was expressed in the soluble form and purified by nickel-affinity chromatography to homogeneity. The amount of S protein recovered was 0.2-0.3mg/100ml bacterial culture. The S protein was recognized by sera from SARS patients by ELISA and Western blot, which indicated that recombinant S protein retained its antigenicity. By biotinylated ELISA and Western blot using biotin-labeled S protein as the probe, we identified 130-kDa and 140-kDa proteins in Vero cells that might be the cellular receptors responsible for SARS-CoV infection. Taken together, these results suggested that recombinant S protein exhibited the antigenicity and receptor-binding ability, and it could be a good candidate for further developing SARS vaccine and anti-SARS therapy." @default.
• W2000615699 created "2016-06-24" @default.
• W2000615699 creator A5011770577 @default.
• W2000615699 creator A5021867307 @default.
• W2000615699 creator A5036090873 @default.
• W2000615699 creator A5052857284 @default.
• W2000615699 creator A5067495209 @default.
• W2000615699 creator A5086215149 @default.
• W2000615699 date "2004-01-01" @default.
• W2000615699 modified "2023-10-03" @default.
• W2000615699 title "Antigenicity and receptor-binding ability of recombinant SARS coronavirus spike protein" @default.
• W2000615699 cites W1526839960 @default.
• W2000615699 cites W1551068445 @default.
• W2000615699 cites W1975304761 @default.
• W2000615699 cites W1976741900 @default.
• W2000615699 cites W1991569048 @default.
• W2000615699 cites W2001745911 @default.
• W2000615699 cites W2010296058 @default.
• W2000615699 cites W2025170735 @default.
• W2000615699 cites W2042858928 @default.
• W2000615699 cites W2046588052 @default.
• W2000615699 cites W2048258437 @default.
• W2000615699 cites W2055708140 @default.
• W2000615699 cites W2080757497 @default.
• W2000615699 cites W2087202599 @default.
• W2000615699 cites W2096771998 @default.
• W2000615699 cites W2097622821 @default.
• W2000615699 cites W2104548316 @default.
• W2000615699 cites W2116586125 @default.
• W2000615699 cites W2122296975 @default.
• W2000615699 cites W2148238685 @default.
• W2000615699 cites W2169198329 @default.
• W2000615699 cites W2182143911 @default.
• W2000615699 cites W75422358 @default.
• W2000615699 cites W2416210798 @default.
• W2000615699 doi "https://doi.org/10.1016/j.bbrc.2003.11.180" @default.
• W2000615699 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7111049" @default.
• W2000615699 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14706633" @default.
• W2000615699 hasPublicationYear "2004" @default.
• W2000615699 type Work @default.
• W2000615699 sameAs 2000615699 @default.
• W2000615699 citedByCount "34" @default.
• W2000615699 countsByYear W20006156992013 @default.
• W2000615699 countsByYear W20006156992014 @default.
• W2000615699 countsByYear W20006156992016 @default.
• W2000615699 countsByYear W20006156992020 @default.
• W2000615699 countsByYear W20006156992022 @default.
• W2000615699 crossrefType "journal-article" @default.
• W2000615699 hasAuthorship W2000615699A5011770577 @default.
• W2000615699 hasAuthorship W2000615699A5021867307 @default.
• W2000615699 hasAuthorship W2000615699A5036090873 @default.
• W2000615699 hasAuthorship W2000615699A5052857284 @default.
• W2000615699 hasAuthorship W2000615699A5067495209 @default.
• W2000615699 hasAuthorship W2000615699A5086215149 @default.
• W2000615699 hasBestOaLocation W20006156991 @default.
• W2000615699 hasConcept C104317684 @default.
• W2000615699 hasConcept C123894998 @default.
• W2000615699 hasConcept C136071829 @default.
• W2000615699 hasConcept C142724271 @default.
• W2000615699 hasConcept C153911025 @default.
• W2000615699 hasConcept C159047783 @default.
• W2000615699 hasConcept C159654299 @default.
• W2000615699 hasConcept C170493617 @default.
• W2000615699 hasConcept C203014093 @default.
• W2000615699 hasConcept C204628709 @default.
• W2000615699 hasConcept C2522874641 @default.
• W2000615699 hasConcept C2776415932 @default.
• W2000615699 hasConcept C2777648638 @default.
• W2000615699 hasConcept C2779026604 @default.
• W2000615699 hasConcept C2779134260 @default.
• W2000615699 hasConcept C3008058167 @default.
• W2000615699 hasConcept C40767141 @default.
• W2000615699 hasConcept C524204448 @default.
• W2000615699 hasConcept C55493867 @default.
• W2000615699 hasConcept C71924100 @default.
• W2000615699 hasConcept C86803240 @default.
• W2000615699 hasConcept C89138240 @default.
• W2000615699 hasConceptScore W2000615699C104317684 @default.
• W2000615699 hasConceptScore W2000615699C123894998 @default.
• W2000615699 hasConceptScore W2000615699C136071829 @default.
• W2000615699 hasConceptScore W2000615699C142724271 @default.
• W2000615699 hasConceptScore W2000615699C153911025 @default.
• W2000615699 hasConceptScore W2000615699C159047783 @default.
• W2000615699 hasConceptScore W2000615699C159654299 @default.
• W2000615699 hasConceptScore W2000615699C170493617 @default.
• W2000615699 hasConceptScore W2000615699C203014093 @default.
• W2000615699 hasConceptScore W2000615699C204628709 @default.
• W2000615699 hasConceptScore W2000615699C2522874641 @default.
• W2000615699 hasConceptScore W2000615699C2776415932 @default.
• W2000615699 hasConceptScore W2000615699C2777648638 @default.
• W2000615699 hasConceptScore W2000615699C2779026604 @default.
• W2000615699 hasConceptScore W2000615699C2779134260 @default.
• W2000615699 hasConceptScore W2000615699C3008058167 @default.
• W2000615699 hasConceptScore W2000615699C40767141 @default.
• W2000615699 hasConceptScore W2000615699C524204448 @default.
• W2000615699 hasConceptScore W2000615699C55493867 @default.
• W2000615699 hasConceptScore W2000615699C71924100 @default.
• W2000615699 hasConceptScore W2000615699C86803240 @default.

• next