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- W2000618441 abstract "The purpose of this study was to characterize the uptake of liposomal phosphatidylcholine by lung tissue and its subcellular organelles. Multilamellar liposomes were prepared from egg yolk phosphatidylcholine, dicetyl phosphate, and cholesterol (molar ratio 7:2:1). Liposomal phosphatidylcholine labeled with [1-14C]dipalmitoyl phosphatidylcholine was taken up by lung slices and incorporated into subcellular organelles including lamellar bodies, mitochondria, and microsomes. In addition, when liposomes were incubated with lamellar bodies, mitochondria, or microsomes, the transfer of liposomal phosphatidylcholine to these subcellular fractions was facilitated by the cytosolic fraction. In tissue slice experiments after 1 h of incubation, about 86% of the total radioactivity absorbed by lung slices and subcellular organelles was recovered in phosphatidylcholine. The ratio of the radioactivity of fatty acids at 1- and 2-positions of dipalmitoyl phosphatidylcholine recovered from all fractions was nearly 1:1. This suggests that most phosphatidylcholine molecules were taken up intact. In conclusion, this study provides a method of using liposomes as a tool for probing the phosphatidylcholine transfer mechanism in lung." @default.
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- W2000618441 date "1979-11-01" @default.
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- W2000618441 title "Use of liposomes in probing the uptake of liposomal phosphatidylcholine by rabbit lung in vitro" @default.
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- W2000618441 doi "https://doi.org/10.1016/0005-2760(79)90025-0" @default.
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