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• W2000619268 endingPage "972" @default.
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• W2000619268 abstract "Atox1 is a human copper (Cu) chaperone with the ferredoxin-like fold that binds Cu(I) via two Cys residues in a M(10)X(11)C(12)X(13)X(14)C(15) motif located in a solvent-exposed loop. Here, we report molecular dynamics simulations that reveal the roles of Met10, Thr11, and Lys60 in Atox1 structural dynamics. Whereas Met10 is conserved in all Atox1 homologues, Thr11 and Lys60 are exchanged for Ser and Tyr in bacteria. From simulations on apo and Cu(I) forms of Met10Ala, Thr11Ala, Lys60Ala, Thr11Ser, and Lys60Tyr variants, we have compared a range of structural and dynamic parameters such as backbone/Cu-loop dynamics, Cys solvent exposure, Cys-Cys distances, and cross-correlated motions. Surprisingly, Atox1 becomes more rigid in the absence of either Thr11 or Lys60, suggesting that these residues introduce protein flexibility. Lys60 and Thr11 also participate in electrostatic networks that stabilize the Cu-bound form and, in the apo form, determine the solvent exposure of the two Cys residues. In contrast, Met10 is buried in the hydrophobic core of Atox1, and its removal results in a dynamic protein structure. Prokaryotic residues are not good substitutes for the eukaryotic counterparts implying early divergence of Cu chaperone homologues. It appears that Atox1 residues have been conserved to ensure backbone/loop flexibility, electrostatic Cu site stabilization, and proper core packing. The discovered built-in flexibility may be directly linked to structural changes needed to form transient Atox1-Cu-target complexes in vivo." @default.
• W2000619268 created "2016-06-24" @default.
• W2000619268 creator A5014890729 @default.
• W2000619268 creator A5063954863 @default.
• W2000619268 date "2009-01-15" @default.
• W2000619268 modified "2023-09-26" @default.
• W2000619268 title "Differential Roles of Met10, Thr11, and Lys60 in Structural Dynamics of Human Copper Chaperone Atox1" @default.
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• W2000619268 doi "https://doi.org/10.1021/bi8018652" @default.
• W2000619268 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19146392" @default.
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