FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000619277> ?p ?o ?g. }

• W2000619277 endingPage "229" @default.
• W2000619277 startingPage "223" @default.
• W2000619277 abstract "Diagnosis of the cutaneous form of canine leishmaniosis is mostly performed by histological or immunohistological examination of skin biopsies. In modern histology, the polymerase chain reaction (PCR) has gained increasing importance as a complementary tool to directly demonstrate the presence of parasite DNA in the tissue sections. For the present study, a previously described Leishmania-PCR has been further developed and optimised in view of its practicability for routine histological application. Since formalin-fixation of histological specimens causes partial DNA-destruction, which may hamper diagnostic PCR analysis, primers specific for the highly conserved α-actin gene sequences were used to pre-diagnostically assess the isolated sample-DNA for its functionality in a PCR-reaction. This α-actin-specific PCR detects DNA from a large variety of mammalian species and thus exhibits relevance for both human and veterinary medical application. A recombinant internal positive control was introduced to monitor possible sample-related inhibitory effects during the amplification reaction. We performed a retrospective evaluative study with 18 formalin-fixed samples from dogs with suspected or proven leishmaniosis. Six samples were PCR-incompatible. In turn, 9 of the other 12 samples were PCR-positive, and immunohistochemical results matched these findings. Based on these technical achievements, the Leishmania-PCR proved to be a valuable tool to complement conventional histological and immunohistological methods for diagnosis of cutaneous leishmaniosis in formalin-fixed, paraffin-embedded skin biopsies." @default.
• W2000619277 created "2016-06-24" @default.
• W2000619277 creator A5009904850 @default.
• W2000619277 creator A5027612465 @default.
• W2000619277 creator A5042127314 @default.
• W2000619277 creator A5053218696 @default.
• W2000619277 creator A5088054072 @default.
• W2000619277 creator A5090969657 @default.
• W2000619277 date "2003-06-01" @default.
• W2000619277 modified "2023-09-27" @default.
• W2000619277 title "PCR-based detection of canine Leishmania infections in formalin-fixed and paraffin-embedded skin biopsies: elaboration of a protocol for quality assessment of the diagnostic amplification reaction" @default.
• W2000619277 cites W1967697216 @default.
• W2000619277 cites W1987176207 @default.
• W2000619277 cites W2017506149 @default.
• W2000619277 cites W2022304552 @default.
• W2000619277 cites W2041140837 @default.
• W2000619277 cites W2042880479 @default.
• W2000619277 cites W2045873956 @default.
• W2000619277 cites W2062344073 @default.
• W2000619277 cites W2066721968 @default.
• W2000619277 cites W2078886897 @default.
• W2000619277 cites W2088152941 @default.
• W2000619277 cites W2139174197 @default.
• W2000619277 cites W2163922400 @default.
• W2000619277 cites W2168287669 @default.
• W2000619277 cites W2168467494 @default.
• W2000619277 doi "https://doi.org/10.1016/s0304-4017(03)00139-0" @default.
• W2000619277 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12788257" @default.
• W2000619277 hasPublicationYear "2003" @default.
• W2000619277 type Work @default.
• W2000619277 sameAs 2000619277 @default.
• W2000619277 citedByCount "74" @default.
• W2000619277 countsByYear W20006192772012 @default.
• W2000619277 countsByYear W20006192772013 @default.
• W2000619277 countsByYear W20006192772014 @default.
• W2000619277 countsByYear W20006192772015 @default.
• W2000619277 countsByYear W20006192772016 @default.
• W2000619277 countsByYear W20006192772017 @default.
• W2000619277 countsByYear W20006192772018 @default.
• W2000619277 countsByYear W20006192772019 @default.
• W2000619277 countsByYear W20006192772020 @default.
• W2000619277 countsByYear W20006192772021 @default.
• W2000619277 countsByYear W20006192772022 @default.
• W2000619277 countsByYear W20006192772023 @default.
• W2000619277 crossrefType "journal-article" @default.
• W2000619277 hasAuthorship W2000619277A5009904850 @default.
• W2000619277 hasAuthorship W2000619277A5027612465 @default.
• W2000619277 hasAuthorship W2000619277A5042127314 @default.
• W2000619277 hasAuthorship W2000619277A5053218696 @default.
• W2000619277 hasAuthorship W2000619277A5088054072 @default.
• W2000619277 hasAuthorship W2000619277A5090969657 @default.
• W2000619277 hasConcept C104317684 @default.
• W2000619277 hasConcept C136764020 @default.
• W2000619277 hasConcept C142724271 @default.
• W2000619277 hasConcept C203014093 @default.
• W2000619277 hasConcept C204232928 @default.
• W2000619277 hasConcept C2775934546 @default.
• W2000619277 hasConcept C2781092759 @default.
• W2000619277 hasConcept C41008148 @default.
• W2000619277 hasConcept C49105822 @default.
• W2000619277 hasConcept C55493867 @default.
• W2000619277 hasConcept C57742111 @default.
• W2000619277 hasConcept C71924100 @default.
• W2000619277 hasConcept C71928629 @default.
• W2000619277 hasConcept C86803240 @default.
• W2000619277 hasConceptScore W2000619277C104317684 @default.
• W2000619277 hasConceptScore W2000619277C136764020 @default.
• W2000619277 hasConceptScore W2000619277C142724271 @default.
• W2000619277 hasConceptScore W2000619277C203014093 @default.
• W2000619277 hasConceptScore W2000619277C204232928 @default.
• W2000619277 hasConceptScore W2000619277C2775934546 @default.
• W2000619277 hasConceptScore W2000619277C2781092759 @default.
• W2000619277 hasConceptScore W2000619277C41008148 @default.
• W2000619277 hasConceptScore W2000619277C49105822 @default.
• W2000619277 hasConceptScore W2000619277C55493867 @default.
• W2000619277 hasConceptScore W2000619277C57742111 @default.
• W2000619277 hasConceptScore W2000619277C71924100 @default.
• W2000619277 hasConceptScore W2000619277C71928629 @default.
• W2000619277 hasConceptScore W2000619277C86803240 @default.
• W2000619277 hasIssue "3" @default.
• W2000619277 hasLocation W20006192771 @default.
• W2000619277 hasLocation W20006192772 @default.
• W2000619277 hasOpenAccess W2000619277 @default.
• W2000619277 hasPrimaryLocation W20006192771 @default.
• W2000619277 hasRelatedWork W1993757192 @default.
• W2000619277 hasRelatedWork W1995347403 @default.
• W2000619277 hasRelatedWork W2009144746 @default.
• W2000619277 hasRelatedWork W2024105453 @default.
• W2000619277 hasRelatedWork W2129443427 @default.
• W2000619277 hasRelatedWork W2153927276 @default.
• W2000619277 hasRelatedWork W2164046806 @default.
• W2000619277 hasRelatedWork W2294970681 @default.
• W2000619277 hasRelatedWork W2408693058 @default.
• W2000619277 hasRelatedWork W4240417676 @default.
• W2000619277 hasVolume "114" @default.
• W2000619277 isParatext "false" @default.
• W2000619277 isRetracted "false" @default.
• W2000619277 magId "2000619277" @default.

• next