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• W2000620225 abstract "Two techniques for transferring biomaterial using a pulsed laser beam were developed: matrix-assisted pulsed laser evaporation (MAPLE) and MAPLE direct write (MDW). MAPLE is a large-area vacuum based technique suitable for coatings, i.e., antibiofouling, and MDW is a localized deposition technique capable of fast prototyping of devices, i.e., protein or tissue arrays. Both techniques have demonstrated the capability of transferring large (mol wt&gt;100 kDa) molecules in different forms, e.g., liquid and gel, and preserving their functions. They can deposit patterned films with spatial accuracy and resolution of tens of μm and layering on a variety of substrate materials and geometries. MDW can dispense volumes less than 100 pl, transfer solid tissues, fabricate a complete device, and is computed aided design/computer aided manufacturing compatible. They are noncontact techniques and can be integrated with other sterile processes. These attributes are substantiated by films and arrays of biomaterials, e.g., polymers, enzymes, proteins, eucaryotic cells, and tissue, and a dopamine sensor. These examples, the instrumentation, basic mechanisms, a comparison with other techniques, and future developments are discussed." @default.
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• W2000620225 date "2003-03-26" @default.
• W2000620225 modified "2023-10-10" @default.
• W2000620225 title "Laser transfer of biomaterials: Matrix-assisted pulsed laser evaporation (MAPLE) and MAPLE Direct Write" @default.
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• W2000620225 doi "https://doi.org/10.1063/1.1544081" @default.
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