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• W2000626804 abstract "ObjectiveTo develop a real-time PCR method, based on the 5′nuclease TaqMan technology, for quantitation of clonal cells in multiple myeloma (MM).Materials and MethodsThe real-time quantitative PCR method incorporates both an allele-specific oligonucleotides (ASO) primer and an ASO dual-labeled fluorogenic probe (ASO TaqMan probe). The ASO primer and probe corresponded to the complementary determining region 3 (CDR3) of the rearranged immunoglobulin heavy chain gene (IgH). With the use of a sequence detector, PCR product accumulation was measured through the ASO TaqMan probe. The real-time PCR method was compared with flow cytometric quantitation of myeloma plasma cells.ResultsThe application of the real-time quantitative ASO IgH PCR method is illustrated by a sequential analysis of minimal residual disease (MRD) in bone marrow (BM) samples from myeloma patients undergoing peripheral blood stem cell (PBSC) transplantation. The real-time PCR method was able to quantitate residual malignant cells in BM samples from patients who were considered to be in complete remission. Further, it was illustrated that a potential problem in determining tumor cell content in myeloma BM samples is the heterogeneous infiltration of the marrow.ConclusionThe application of the real-time PCR method provides a sensitive, highly specific, and reproducible quantitation of myeloma cells." @default.
• W2000626804 created "2016-06-24" @default.
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• W2000626804 date "2000-09-01" @default.
• W2000626804 modified "2023-10-15" @default.
• W2000626804 title "Quantitation of minimal residual disease in multiple myeloma using an allele-specific real-time PCR assay" @default.
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• W2000626804 doi "https://doi.org/10.1016/s0301-472x(00)00514-2" @default.
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