FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000628323> ?p ?o ?g. }

• W2000628323 endingPage "1045" @default.
• W2000628323 startingPage "1039" @default.
• W2000628323 abstract "ObjectiveIn vitro expansion is an important step to acquire sufficient cells in human tissue engineering technologies. The high number of chondrocytes needed for human articular cartilage implants requires in vitro expansion of the primary cells, bearing a theoretical risk of in vitro induced changes in the genomes. To gain more insights into this situation, model cultures were prepared and analyzed.Design25 chondrocyte cell DNA samples from nine donors were analyzed by array comparative genomic hybridization (aCGH) on whole genome level and 28 chondrocyte cell samples from 16 individuals were analyzed by fluorescence in situ hybridization (FISH) on single cell level. The expanded cells were further characterized upon the chondrocytic mRNA phenotype by reverse-transciptase polymerase chain reaction (RT-PCR).ResultsThe molecular karyotyping results revealed autosomal stability, but all male samples analyzed by aCGH displayed a variable loss of the Y-chromosome. These data were confirmed by FISH-experiments and suggest an age dependant effect toward the loss of the Y-chromosome in cultured chondrocytes. RT-PCR data for the mRNAs from collagen types I, II, and aggrecan and the pro-inflammatory cytokine interleukin-1ß (IL-1ß) did not reveal any correlation of transcriptional activity in cultures with Y-chromosome losses, nor were there statistically significant differences between cells from female and male donors.ConclusionsWhile cells of male origin may suffer from an age-related loss of the Y-chromosome, there was no indication of a functional impairment. The data suggest some caution toward applying proliferative steps when considering chondrocytes from elderly male patients for tissue engineering approaches. In vitro expansion is an important step to acquire sufficient cells in human tissue engineering technologies. The high number of chondrocytes needed for human articular cartilage implants requires in vitro expansion of the primary cells, bearing a theoretical risk of in vitro induced changes in the genomes. To gain more insights into this situation, model cultures were prepared and analyzed. 25 chondrocyte cell DNA samples from nine donors were analyzed by array comparative genomic hybridization (aCGH) on whole genome level and 28 chondrocyte cell samples from 16 individuals were analyzed by fluorescence in situ hybridization (FISH) on single cell level. The expanded cells were further characterized upon the chondrocytic mRNA phenotype by reverse-transciptase polymerase chain reaction (RT-PCR). The molecular karyotyping results revealed autosomal stability, but all male samples analyzed by aCGH displayed a variable loss of the Y-chromosome. These data were confirmed by FISH-experiments and suggest an age dependant effect toward the loss of the Y-chromosome in cultured chondrocytes. RT-PCR data for the mRNAs from collagen types I, II, and aggrecan and the pro-inflammatory cytokine interleukin-1ß (IL-1ß) did not reveal any correlation of transcriptional activity in cultures with Y-chromosome losses, nor were there statistically significant differences between cells from female and male donors. While cells of male origin may suffer from an age-related loss of the Y-chromosome, there was no indication of a functional impairment. The data suggest some caution toward applying proliferative steps when considering chondrocytes from elderly male patients for tissue engineering approaches." @default.
• W2000628323 created "2016-06-24" @default.
• W2000628323 creator A5005660033 @default.
• W2000628323 creator A5030909606 @default.
• W2000628323 creator A5040550572 @default.
• W2000628323 creator A5047236675 @default.
• W2000628323 creator A5050356182 @default.
• W2000628323 creator A5069454779 @default.
• W2000628323 creator A5089432773 @default.
• W2000628323 date "2012-09-01" @default.
• W2000628323 modified "2023-09-27" @default.
• W2000628323 title "Genomic chondrocyte culture profiling by array-CGH, interphase-FISH and RT-PCR" @default.
• W2000628323 cites W1968116037 @default.
• W2000628323 cites W1969252625 @default.
• W2000628323 cites W2008721567 @default.
• W2000628323 cites W2014275733 @default.
• W2000628323 cites W2041591102 @default.
• W2000628323 cites W2042287926 @default.
• W2000628323 cites W2047019014 @default.
• W2000628323 cites W2056041841 @default.
• W2000628323 cites W2061386274 @default.
• W2000628323 cites W2071147330 @default.
• W2000628323 cites W2077969224 @default.
• W2000628323 cites W2103252028 @default.
• W2000628323 cites W2108192088 @default.
• W2000628323 cites W2108434819 @default.
• W2000628323 cites W2123405901 @default.
• W2000628323 cites W2129685860 @default.
• W2000628323 cites W2141588974 @default.
• W2000628323 cites W4238966960 @default.
• W2000628323 cites W4366080542 @default.
• W2000628323 doi "https://doi.org/10.1016/j.joca.2012.05.012" @default.
• W2000628323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22698443" @default.
• W2000628323 hasPublicationYear "2012" @default.
• W2000628323 type Work @default.
• W2000628323 sameAs 2000628323 @default.
• W2000628323 citedByCount "8" @default.
• W2000628323 countsByYear W20006283232016 @default.
• W2000628323 countsByYear W20006283232020 @default.
• W2000628323 countsByYear W20006283232021 @default.
• W2000628323 countsByYear W20006283232022 @default.
• W2000628323 crossrefType "journal-article" @default.
• W2000628323 hasAuthorship W2000628323A5005660033 @default.
• W2000628323 hasAuthorship W2000628323A5030909606 @default.
• W2000628323 hasAuthorship W2000628323A5040550572 @default.
• W2000628323 hasAuthorship W2000628323A5047236675 @default.
• W2000628323 hasAuthorship W2000628323A5050356182 @default.
• W2000628323 hasAuthorship W2000628323A5069454779 @default.
• W2000628323 hasAuthorship W2000628323A5089432773 @default.
• W2000628323 hasBestOaLocation W20006283231 @default.
• W2000628323 hasConcept C104317684 @default.
• W2000628323 hasConcept C105580179 @default.
• W2000628323 hasConcept C124942203 @default.
• W2000628323 hasConcept C142724271 @default.
• W2000628323 hasConcept C153911025 @default.
• W2000628323 hasConcept C197534660 @default.
• W2000628323 hasConcept C202751555 @default.
• W2000628323 hasConcept C204787440 @default.
• W2000628323 hasConcept C2776164576 @default.
• W2000628323 hasConcept C2777542201 @default.
• W2000628323 hasConcept C2781403057 @default.
• W2000628323 hasConcept C3020332539 @default.
• W2000628323 hasConcept C30481170 @default.
• W2000628323 hasConcept C53226629 @default.
• W2000628323 hasConcept C54355233 @default.
• W2000628323 hasConcept C71924100 @default.
• W2000628323 hasConcept C81439078 @default.
• W2000628323 hasConcept C86803240 @default.
• W2000628323 hasConceptScore W2000628323C104317684 @default.
• W2000628323 hasConceptScore W2000628323C105580179 @default.
• W2000628323 hasConceptScore W2000628323C124942203 @default.
• W2000628323 hasConceptScore W2000628323C142724271 @default.
• W2000628323 hasConceptScore W2000628323C153911025 @default.
• W2000628323 hasConceptScore W2000628323C197534660 @default.
• W2000628323 hasConceptScore W2000628323C202751555 @default.
• W2000628323 hasConceptScore W2000628323C204787440 @default.
• W2000628323 hasConceptScore W2000628323C2776164576 @default.
• W2000628323 hasConceptScore W2000628323C2777542201 @default.
• W2000628323 hasConceptScore W2000628323C2781403057 @default.
• W2000628323 hasConceptScore W2000628323C3020332539 @default.
• W2000628323 hasConceptScore W2000628323C30481170 @default.
• W2000628323 hasConceptScore W2000628323C53226629 @default.
• W2000628323 hasConceptScore W2000628323C54355233 @default.
• W2000628323 hasConceptScore W2000628323C71924100 @default.
• W2000628323 hasConceptScore W2000628323C81439078 @default.
• W2000628323 hasConceptScore W2000628323C86803240 @default.
• W2000628323 hasIssue "9" @default.
• W2000628323 hasLocation W20006283231 @default.
• W2000628323 hasLocation W20006283232 @default.
• W2000628323 hasOpenAccess W2000628323 @default.
• W2000628323 hasPrimaryLocation W20006283231 @default.
• W2000628323 hasRelatedWork W1975039090 @default.
• W2000628323 hasRelatedWork W2001269759 @default.
• W2000628323 hasRelatedWork W2045840608 @default.
• W2000628323 hasRelatedWork W2049235446 @default.
• W2000628323 hasRelatedWork W2056275775 @default.
• W2000628323 hasRelatedWork W2079203029 @default.
• W2000628323 hasRelatedWork W2083495987 @default.

• next