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- W2000636004 abstract "From the mixture of 4(2)-O-beta-D-galactosyl-maltose (Gal-G2) and beta-cyclodextrin (betaCD), novel heterobranched betaCDs, (Gal-G2)-betaCD and (Gal-G2)2-betaCDs, were synthesized by the reverse action of debranching enzyme. The optimum conditions for the production of (Gal-G2)2-betaCDs were examined. A mixture of (Gal-G2)2-betaCDs was produced in about 4% yield when Aerobacter aerogenes pullulanase (64 units per 1 g of Gal-G2) was incubated with 1.6 M Gal-G2 and 0.16 M betaCD at 50 degrees C for 4 days. The reaction products, (Gal-G2)2-betaCDs, were separated into three peaks by HPLC analysis on a Hypercarb S column. Their structures were analyzed by fast atom bombardment mass spectroscopy and NMR spectroscopies, and confirmed by comparison of their hydrolyzates by beta-galactosidase with the authentic (G2)2 -betaCDs. The structures of (Gal-G2)-betaCD and three components of (Gal-G2)2-betaCDs were identified as 6-O-(GalG2)-betaCD, 6(1),6(2)-, 6(1),6(3)- and 6(1),6(4)-di-O-(Gal-G2)2-betaCD, respectively." @default.
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- W2000636004 date "2000-01-01" @default.
- W2000636004 modified "2023-09-27" @default.
- W2000636004 title "Synthesis of Novel Heterobranched β-Cyclodextrins from 4<sup>2</sup>-<i>O</i>-β-<scp>D</scp>-Galactosyl- Maltose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products" @default.
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- W2000636004 doi "https://doi.org/10.1271/bbb.64.1223" @default.
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