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Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000640512> ?p ?o ?g. }

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• W2000640512 abstract "Upon exposure to excess glucose, respiring cultures of Saccharomyces cerevisiae produce substantial amounts of ethanol and acetate. A possible role of a limited anaplerotic capacity in this process was investigated by overexpressing pyruvate carboxylase and by replacing it with a heterologous enzyme (Escherichia coli phosphoenolpyruvate carboxylase). Compared to the wild-type, neither the pyruvate carboxylase (Pyc)-overexpressing nor the transgenic strain exhibited reduced by-product formation after glucose pulses to aerobic glucose-limited chemostat cultures. An increased intracellular malate concentration was observed in the two engineered strains. It is concluded that by-product formation in S. cerevisiae is not caused by a limited anaplerotic capacity." @default.
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• W2000640512 date "1999-10-01" @default.
• W2000640512 modified "2023-10-07" @default.
• W2000640512 title "By-product formation during exposure of respiringSaccharomyces cerevisiaecultures to excess glucose is not caused by a limited capacity of pyruvate carboxylase" @default.
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• W2000640512 doi "https://doi.org/10.1111/j.1574-6968.1999.tb08715.x" @default.
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