FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000640600> ?p ?o ?g. }

• W2000640600 endingPage "9259" @default.
• W2000640600 startingPage "9251" @default.
• W2000640600 abstract "Several electrospray-mass spectrometry (ESI-MS)-based methods are available for determining the constant of association (K(a)) between a protein and a small ligand, but current MS-based strategies are not fully adequate for measuring K(a) of protein-protein interactions accurately. We expanded the application of ESI-MS-based titration to determine the strength of noncovalent interactions between proteins, forming a complex. Taking into account relative response factors (probability of being ionized, transmitted, and detected), we determined K(a) values of an equilibrium between dimers and tetramers at three different pH values (6.8, 3.4, and 8.4). We investigated the association of the lectin concanavalin A, whose dimer-tetramer ratio in the gas phase is affected by solution concentration and by pH. To calculate the constants of association in solution, we also utilized isothermal titration calorimetry (ITC) for a comparison with MS-based titration. At pH 6.8 and pH 8.4, the K(a) values measured by MS and by ITC were in agreement. ITC results allowed us to restrain the response factor to a value close to 4. At pH 3.4, we were able to measure the K(a) only by MS, but not by ITC because of limited sensitivity of calorimetry. Our investigation illustrates the great potential MS for calculating the binding strength of protein-protein interactions within noncovalent complexes. The main advantages of MS over ITC are its sensitivity (i.e., the required amount of sample is >100 times less than the one necessary for ITC), and the possibility to obtain precise information on composition of protein complexes, their stoichiometry, their subunit interactions, and their assembly pathway. Compared to previous investigations, our study shows the strong influence of response factors on determining accurate protein-protein association constants by MS." @default.
• W2000640600 created "2016-06-24" @default.
• W2000640600 creator A5001571695 @default.
• W2000640600 creator A5004688568 @default.
• W2000640600 creator A5019979618 @default.
• W2000640600 creator A5049692788 @default.
• W2000640600 date "2011-11-16" @default.
• W2000640600 modified "2023-09-23" @default.
• W2000640600 title "Quantifying Protein–Protein Interactions Within Noncovalent Complexes Using Electrospray Ionization Mass Spectrometry" @default.
• W2000640600 cites W1574727640 @default.
• W2000640600 cites W1966480833 @default.
• W2000640600 cites W1980949887 @default.
• W2000640600 cites W1983785284 @default.
• W2000640600 cites W1985079632 @default.
• W2000640600 cites W1986521396 @default.
• W2000640600 cites W1990219361 @default.
• W2000640600 cites W1991540499 @default.
• W2000640600 cites W1992493959 @default.
• W2000640600 cites W1993064699 @default.
• W2000640600 cites W2008339646 @default.
• W2000640600 cites W2009088712 @default.
• W2000640600 cites W2019072196 @default.
• W2000640600 cites W2019968440 @default.
• W2000640600 cites W2028592651 @default.
• W2000640600 cites W2030928323 @default.
• W2000640600 cites W2034614180 @default.
• W2000640600 cites W2040809710 @default.
• W2000640600 cites W2053234014 @default.
• W2000640600 cites W2066011752 @default.
• W2000640600 cites W2067326301 @default.
• W2000640600 cites W2071884490 @default.
• W2000640600 cites W2075219502 @default.
• W2000640600 cites W2076027254 @default.
• W2000640600 cites W2100043909 @default.
• W2000640600 cites W2130213960 @default.
• W2000640600 cites W2147990271 @default.
• W2000640600 cites W2160794341 @default.
• W2000640600 cites W2409678684 @default.
• W2000640600 doi "https://doi.org/10.1021/ac201576e" @default.
• W2000640600 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22047453" @default.
• W2000640600 hasPublicationYear "2011" @default.
• W2000640600 type Work @default.
• W2000640600 sameAs 2000640600 @default.
• W2000640600 citedByCount "50" @default.
• W2000640600 countsByYear W20006406002012 @default.
• W2000640600 countsByYear W20006406002013 @default.
• W2000640600 countsByYear W20006406002014 @default.
• W2000640600 countsByYear W20006406002015 @default.
• W2000640600 countsByYear W20006406002016 @default.
• W2000640600 countsByYear W20006406002017 @default.
• W2000640600 countsByYear W20006406002018 @default.
• W2000640600 countsByYear W20006406002019 @default.
• W2000640600 countsByYear W20006406002020 @default.
• W2000640600 countsByYear W20006406002021 @default.
• W2000640600 countsByYear W20006406002022 @default.
• W2000640600 countsByYear W20006406002023 @default.
• W2000640600 crossrefType "journal-article" @default.
• W2000640600 hasAuthorship W2000640600A5001571695 @default.
• W2000640600 hasAuthorship W2000640600A5004688568 @default.
• W2000640600 hasAuthorship W2000640600A5019979618 @default.
• W2000640600 hasAuthorship W2000640600A5049692788 @default.
• W2000640600 hasConcept C112887158 @default.
• W2000640600 hasConcept C113196181 @default.
• W2000640600 hasConcept C137277065 @default.
• W2000640600 hasConcept C147789679 @default.
• W2000640600 hasConcept C156911925 @default.
• W2000640600 hasConcept C162356407 @default.
• W2000640600 hasConcept C178790620 @default.
• W2000640600 hasConcept C181199279 @default.
• W2000640600 hasConcept C184866935 @default.
• W2000640600 hasConcept C185592680 @default.
• W2000640600 hasConcept C194451485 @default.
• W2000640600 hasConcept C2778886173 @default.
• W2000640600 hasConcept C2779546866 @default.
• W2000640600 hasConcept C32909587 @default.
• W2000640600 hasConcept C40684141 @default.
• W2000640600 hasConcept C43617362 @default.
• W2000640600 hasConcept C8010536 @default.
• W2000640600 hasConceptScore W2000640600C112887158 @default.
• W2000640600 hasConceptScore W2000640600C113196181 @default.
• W2000640600 hasConceptScore W2000640600C137277065 @default.
• W2000640600 hasConceptScore W2000640600C147789679 @default.
• W2000640600 hasConceptScore W2000640600C156911925 @default.
• W2000640600 hasConceptScore W2000640600C162356407 @default.
• W2000640600 hasConceptScore W2000640600C178790620 @default.
• W2000640600 hasConceptScore W2000640600C181199279 @default.
• W2000640600 hasConceptScore W2000640600C184866935 @default.
• W2000640600 hasConceptScore W2000640600C185592680 @default.
• W2000640600 hasConceptScore W2000640600C194451485 @default.
• W2000640600 hasConceptScore W2000640600C2778886173 @default.
• W2000640600 hasConceptScore W2000640600C2779546866 @default.
• W2000640600 hasConceptScore W2000640600C32909587 @default.
• W2000640600 hasConceptScore W2000640600C40684141 @default.
• W2000640600 hasConceptScore W2000640600C43617362 @default.
• W2000640600 hasConceptScore W2000640600C8010536 @default.
• W2000640600 hasIssue "24" @default.
• W2000640600 hasLocation W20006406001 @default.
• W2000640600 hasLocation W20006406002 @default.

• next