FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000642498> ?p ?o ?g. }

• W2000642498 endingPage "1416" @default.
• W2000642498 startingPage "1406" @default.
• W2000642498 abstract "A major pathway for chronic ethanol-induced liver injury is ethanol-induced oxidant stress. Several pathways contribute to mechanisms by which ethanol induces oxidant stress. Although some studies support a role for cytochrome P450 2E1 (CYP2E1), others do not. Most previous studies were conducted in the intragastric infusion model of ethanol administration. There is a need to develop oral models of significant liver injury and to evaluate the possible role of CYP2E1 in ethanol actions in such models. We evaluated chronic ethanol-induced liver injury, steatosis, and oxidant stress in wild-type (WT) mice, CYP2E1 knock out (KO) mice, and humanized CYP2E1 knock-in (KI) mice, in which the human 2E1 was added back to mice deficient in the mouse 2E1. WT mice and the CYP2E1 KO and KI mice (both provided by Dr. F. Gonzalez, National Cancer Institute) were fed a high-fat Lieber–DeCarli ethanol liquid diet for 3 weeks; pair-fed controls received dextrose. Ethanol produced fatty liver and oxidant stress in WT mice but liver injury (transaminases, histopathology) was minimal. Ethanol-induced steatosis and oxidant stress were blunted in the KO mice (no liver injury) but restored in the KI mice. Significant liver injury was produced in the ethanol-fed KI mice, with elevated transaminases, necrosis, and increased levels of collagen type 1 and smooth muscle actin. This liver injury in the KI mice was associated with elevated oxidant stress and elevated levels of the human CYP2E1 compared to levels of the mouse 2E1 in WT mice. Activation of JNK and decreased levels of Bcl-2 and Bcl-XL were observed in the ethanol-fed KI mice compared to the other groups. Fatty liver in the WT and the KI mice was associated with lower levels of PPARα and acyl-CoA oxidase. No such changes were found in the ethanol-fed KO mice. These results show that CYP2E1 plays a major role in ethanol-induced fatty liver and oxidant stress. It is the absence of CYP2E1 in the KO mice that is responsible for the blunting of steatosis and oxidant stress because restoring the CYP2E1 restores the fatty liver and oxidant stress. Moreover, it is the human CYP2E1 that restores these effects of ethanol, which suggests that results for fatty liver and oxidant stress from rodent models of ethanol intake and mouse CYP2E1 can be extrapolated to human models of ethanol intake and to human CYP2E1." @default.
• W2000642498 created "2016-06-24" @default.
• W2000642498 creator A5000536506 @default.
• W2000642498 creator A5008947757 @default.
• W2000642498 creator A5039170596 @default.
• W2000642498 creator A5040205022 @default.
• W2000642498 creator A5046167919 @default.
• W2000642498 date "2010-11-15" @default.
• W2000642498 modified "2023-09-24" @default.
• W2000642498 title "Chronic alcohol-induced liver injury and oxidant stress are decreased in cytochrome P4502E1 knockout mice and restored in humanized cytochrome P4502E1 knock-in mice" @default.
• W2000642498 cites W1543558763 @default.
• W2000642498 cites W1578466218 @default.
• W2000642498 cites W1580569750 @default.
• W2000642498 cites W1768373811 @default.
• W2000642498 cites W1823288226 @default.
• W2000642498 cites W182694654 @default.
• W2000642498 cites W1830134817 @default.
• W2000642498 cites W1902364422 @default.
• W2000642498 cites W1969509136 @default.
• W2000642498 cites W1969677255 @default.
• W2000642498 cites W1971033557 @default.
• W2000642498 cites W1974580919 @default.
• W2000642498 cites W1975192528 @default.
• W2000642498 cites W1981245425 @default.
• W2000642498 cites W1983003002 @default.
• W2000642498 cites W1990413063 @default.
• W2000642498 cites W1992058399 @default.
• W2000642498 cites W1993677761 @default.
• W2000642498 cites W1997037955 @default.
• W2000642498 cites W2009914744 @default.
• W2000642498 cites W2013161374 @default.
• W2000642498 cites W2017421642 @default.
• W2000642498 cites W2020778772 @default.
• W2000642498 cites W2021707548 @default.
• W2000642498 cites W2022763702 @default.
• W2000642498 cites W2029114762 @default.
• W2000642498 cites W2033522281 @default.
• W2000642498 cites W2033689665 @default.
• W2000642498 cites W2034717859 @default.
• W2000642498 cites W2040552715 @default.
• W2000642498 cites W2041379947 @default.
• W2000642498 cites W2041729389 @default.
• W2000642498 cites W2048278498 @default.
• W2000642498 cites W2051096835 @default.
• W2000642498 cites W2053897655 @default.
• W2000642498 cites W2054056570 @default.
• W2000642498 cites W2054511859 @default.
• W2000642498 cites W2055020668 @default.
• W2000642498 cites W2058843590 @default.
• W2000642498 cites W2060452153 @default.
• W2000642498 cites W2061361002 @default.
• W2000642498 cites W2062366543 @default.
• W2000642498 cites W2062398703 @default.
• W2000642498 cites W2066238144 @default.
• W2000642498 cites W2071927516 @default.
• W2000642498 cites W2083511523 @default.
• W2000642498 cites W2093289889 @default.
• W2000642498 cites W2096627428 @default.
• W2000642498 cites W2096764074 @default.
• W2000642498 cites W2100159014 @default.
• W2000642498 cites W2101982676 @default.
• W2000642498 cites W2114111268 @default.
• W2000642498 cites W2122998222 @default.
• W2000642498 cites W2123420560 @default.
• W2000642498 cites W2126568409 @default.
• W2000642498 cites W2130191536 @default.
• W2000642498 cites W2134184179 @default.
• W2000642498 cites W2135047848 @default.
• W2000642498 cites W2137577984 @default.
• W2000642498 cites W2138738670 @default.
• W2000642498 cites W2139847355 @default.
• W2000642498 cites W2142081783 @default.
• W2000642498 cites W2142349229 @default.
• W2000642498 cites W2153023641 @default.
• W2000642498 cites W2164180982 @default.
• W2000642498 cites W2164514101 @default.
• W2000642498 cites W2164794447 @default.
• W2000642498 cites W2170321705 @default.
• W2000642498 cites W2170798897 @default.
• W2000642498 cites W2171969276 @default.
• W2000642498 cites W2172281772 @default.
• W2000642498 cites W2188733915 @default.
• W2000642498 cites W2257536788 @default.
• W2000642498 cites W2979216016 @default.
• W2000642498 cites W4404447 @default.
• W2000642498 cites W2346141041 @default.
• W2000642498 doi "https://doi.org/10.1016/j.freeradbiomed.2010.07.026" @default.
• W2000642498 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2975513" @default.
• W2000642498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20692331" @default.
• W2000642498 hasPublicationYear "2010" @default.
• W2000642498 type Work @default.
• W2000642498 sameAs 2000642498 @default.
• W2000642498 citedByCount "165" @default.
• W2000642498 countsByYear W20006424982012 @default.
• W2000642498 countsByYear W20006424982013 @default.
• W2000642498 countsByYear W20006424982014 @default.
• W2000642498 countsByYear W20006424982015 @default.
• W2000642498 countsByYear W20006424982016 @default.

• next