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- W2000647913 abstract "The dependence of macroscopic gap junctional conductance (gj) upon transjunctional voltage (Vj) was examined in 39 paired osteoblast-like (OB) cells from primary cultures using the double whole cell patch clamp technique. OB cells were derived from calvarial explants of new-born rats. Instantaneous current–voltage (Ij–Vj) relationships of OB cell pairs (n=6) were linear in the entire voltage range (−150≤Vj≤150 mV) examined. The steady-state Ij–Vj relationship was non-linear for Vj≥±60 mV. The curve for the normalised steady-state junctional conductance–voltage relationship (Gss/G0–Vj) was bell-shaped, and was fitted with a two-state Boltzmann equation with a minimum conductance (Gmin) of 0.2–0.3, and a half deactivation voltage (Vo) of ±83 mV. In two recordings unitary gap junction channel activity was observable. The linear I–V relationships revealed a single channel conductance of ∼100 pS. Application of parathyroid hormone (10−8 M) had no effect on the voltage dependence nor the magnitude of macroscopic currents (n=7)." @default.
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- W2000647913 date "1997-07-01" @default.
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- W2000647913 title "Voltage sensitivity of gap junction currents in rat osteoblast-like cells" @default.
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- W2000647913 doi "https://doi.org/10.1016/s0005-2736(97)00048-5" @default.
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