FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000649281> ?p ?o ?g. }

• W2000649281 endingPage "2309" @default.
• W2000649281 startingPage "2301" @default.
• W2000649281 abstract "A circulating tumor cell (CTC) selection microfluidic device was integrated to an electrokinetic enrichment device for preconcentrating CTCs directly from whole blood to allow for the detection of mutations contained within the genomic DNA of the CTCs. Molecular profiling of CTCs can provide important clinical information that cannot be garnered simply by enumerating the selected CTCs. We evaluated our approach using SW620 and HT29 cells (colorectal cancer cell lines) seeded into whole blood as a model system. Because SW620 and HT29 cells overexpress the integral membrane protein EpCAM, they could be immunospecifically selected using a microfluidic device containing anti-EpCAM antibodies immobilized to the walls of a selection bed. The microfluidic device was operated at an optimized flow rate of 2 mm s−1, which allowed for the ability to process 1 mL of whole blood in <40 min. The selected CTCs were then enzymatically released from the antibody selection surface and hydrodynamically transported through a pair of Pt electrodes for conductivity-based enumeration. The efficiency of CTC selection was found to be 96% ± 4%. Following enumeration, the CTCs were hydrodynamically transported at a flow rate of 1 μL min−1 to an on-chip electromanipulation unit, where they were electrophoretically withdrawn from the bulk hydrodynamic flow and directed into a receiving reservoir. Using an electric field of 100 V cm−1, the negatively charged CTCs were enriched into an anodic receiving reservoir to a final volume of 2 μL, providing an enrichment factor of 500. The collected CTCs could then be searched for point mutations using a PCR/LDR/capillary electrophoresis assay. The DNA extracted from the CTCs was subjected to a primary polymerase chain reaction (PCR) with the amplicons used for a ligase detection reaction (LDR) to probe for KRAS oncogenic point mutations. Point mutations in codon 12 of the KRAS gene were successfully detected in the SW620 CTCs for samples containing <10 CTCs in 1 mL of whole blood. However, the HT29 cells did not contain these mutations, consistent with their known genotype." @default.
• W2000649281 created "2016-06-24" @default.
• W2000649281 creator A5026819500 @default.
• W2000649281 creator A5029736335 @default.
• W2000649281 creator A5029953346 @default.
• W2000649281 creator A5038208939 @default.
• W2000649281 creator A5052067814 @default.
• W2000649281 creator A5076833438 @default.
• W2000649281 creator A5086356988 @default.
• W2000649281 creator A5090794208 @default.
• W2000649281 date "2011-02-14" @default.
• W2000649281 modified "2023-09-27" @default.
• W2000649281 title "High-Throughput Selection, Enumeration, Electrokinetic Manipulation, and Molecular Profiling of Low-Abundance Circulating Tumor Cells Using a Microfluidic System" @default.
• W2000649281 cites W1968562444 @default.
• W2000649281 cites W1968700783 @default.
• W2000649281 cites W1974634560 @default.
• W2000649281 cites W1984633251 @default.
• W2000649281 cites W1985942450 @default.
• W2000649281 cites W1988401045 @default.
• W2000649281 cites W1988920364 @default.
• W2000649281 cites W1995249314 @default.
• W2000649281 cites W2002044576 @default.
• W2000649281 cites W2002286265 @default.
• W2000649281 cites W2007592621 @default.
• W2000649281 cites W2010754117 @default.
• W2000649281 cites W2013827382 @default.
• W2000649281 cites W2035037158 @default.
• W2000649281 cites W2044831051 @default.
• W2000649281 cites W2046686567 @default.
• W2000649281 cites W2048452946 @default.
• W2000649281 cites W2050547995 @default.
• W2000649281 cites W2050827942 @default.
• W2000649281 cites W2053928920 @default.
• W2000649281 cites W2069386014 @default.
• W2000649281 cites W2075116234 @default.
• W2000649281 cites W2079698799 @default.
• W2000649281 cites W2081807684 @default.
• W2000649281 cites W2084445306 @default.
• W2000649281 cites W2087398484 @default.
• W2000649281 cites W2088558481 @default.
• W2000649281 cites W2090383796 @default.
• W2000649281 cites W2105381262 @default.
• W2000649281 cites W2111976640 @default.
• W2000649281 cites W2116483002 @default.
• W2000649281 cites W2118835794 @default.
• W2000649281 cites W2128989044 @default.
• W2000649281 cites W2148044748 @default.
• W2000649281 cites W2153557899 @default.
• W2000649281 doi "https://doi.org/10.1021/ac103172y" @default.
• W2000649281 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4380022" @default.
• W2000649281 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21319808" @default.
• W2000649281 hasPublicationYear "2011" @default.
• W2000649281 type Work @default.
• W2000649281 sameAs 2000649281 @default.
• W2000649281 citedByCount "169" @default.
• W2000649281 countsByYear W20006492812012 @default.
• W2000649281 countsByYear W20006492812013 @default.
• W2000649281 countsByYear W20006492812014 @default.
• W2000649281 countsByYear W20006492812015 @default.
• W2000649281 countsByYear W20006492812016 @default.
• W2000649281 countsByYear W20006492812017 @default.
• W2000649281 countsByYear W20006492812018 @default.
• W2000649281 countsByYear W20006492812019 @default.
• W2000649281 countsByYear W20006492812020 @default.
• W2000649281 countsByYear W20006492812021 @default.
• W2000649281 countsByYear W20006492812022 @default.
• W2000649281 countsByYear W20006492812023 @default.
• W2000649281 crossrefType "journal-article" @default.
• W2000649281 hasAuthorship W2000649281A5026819500 @default.
• W2000649281 hasAuthorship W2000649281A5029736335 @default.
• W2000649281 hasAuthorship W2000649281A5029953346 @default.
• W2000649281 hasAuthorship W2000649281A5038208939 @default.
• W2000649281 hasAuthorship W2000649281A5052067814 @default.
• W2000649281 hasAuthorship W2000649281A5076833438 @default.
• W2000649281 hasAuthorship W2000649281A5086356988 @default.
• W2000649281 hasAuthorship W2000649281A5090794208 @default.
• W2000649281 hasBestOaLocation W20006492812 @default.
• W2000649281 hasConcept C104317684 @default.
• W2000649281 hasConcept C114614502 @default.
• W2000649281 hasConcept C121608353 @default.
• W2000649281 hasConcept C147789679 @default.
• W2000649281 hasConcept C153911025 @default.
• W2000649281 hasConcept C156340839 @default.
• W2000649281 hasConcept C1621761 @default.
• W2000649281 hasConcept C171250308 @default.
• W2000649281 hasConcept C185592680 @default.
• W2000649281 hasConcept C192562407 @default.
• W2000649281 hasConcept C203014093 @default.
• W2000649281 hasConcept C27703432 @default.
• W2000649281 hasConcept C2779013556 @default.
• W2000649281 hasConcept C33923547 @default.
• W2000649281 hasConcept C43617362 @default.
• W2000649281 hasConcept C49105822 @default.
• W2000649281 hasConcept C54355233 @default.
• W2000649281 hasConcept C55493867 @default.
• W2000649281 hasConcept C61238886 @default.
• W2000649281 hasConcept C8185291 @default.
• W2000649281 hasConcept C8673954 @default.

• next