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- W2000651315 abstract "The de novo integration of retroviral genomes within the mammalian genome is believed to contribute to the tumorigenic process. Integration may result in the disruption or inappropriate transcription of key regulatory genes. We describe the application of an arbitrarily primed PCR method for the mapping and cloning of genomic integration sites of the mouse mammary tumor virus (MMTV). We have amplified DNA sequences between a selected retroviral MMTV-LTR and random sites in the 3' flanking DNA. Using this technique we were able to visualize several proviral integration sites present in a MMTV-induced mammary tumor derived cell line that were absent from the germ line. Cloning and sequencing of the PCR product corresponding to one site established its identification as an unique 3' flanking sequence." @default.
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- W2000651315 date "1998-04-01" @default.
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- W2000651315 title "Mapping of a mouse mammary tumor virus integration site by retroviral LTR—arbitrary polymerase chain reaction" @default.
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- W2000651315 doi "https://doi.org/10.1016/s0168-1702(98)00002-1" @default.
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