FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000653179> ?p ?o ?g. }

• W2000653179 endingPage "18139" @default.
• W2000653179 startingPage "18134" @default.
• W2000653179 abstract "Hemopoietic cells respond to cytokines by initiating tyrosine phosphorylation of receptors and receptor-associated proteins, leading to the activation of numerous cytosolic and membrane associated enzymes, including phosphatidylinositol 3-OH kinase (PI 3-kinase). Recent reports have suggested that PI 3-kinase may serve as an upstream activator of mitogen-activated protein (MAP) kinase. After stimulation with interleukin-3 and granulocyte-macrophage colony-stimulating factor, we show here that inhibition of MAP kinase activity by two inhibitors of PI 3-kinase, wortmannin and LY-294002, does not correlate with their ability to inhibit PI 3-kinase or p70 S6 kinase phosphorylation. Complete inhibition of phosphatidylinositol 3,4,5-trisphosphate production occurred at approximately 100 nM WM or 25 µM LY-294002, but at these concentrations, WM significantly inhibited MAP kinase activation, while LY-294002 had virtually no effect on MAP kinase activity. Furthermore, WM does not inhibit phorbol ester-mediated MAP kinase activation, but LY-294002 does. Together these results suggest WM and LY-294002 are differentially inhibiting enzymes other than PI 3-kinase that function upstream of MAP kinase. Hemopoietic cells respond to cytokines by initiating tyrosine phosphorylation of receptors and receptor-associated proteins, leading to the activation of numerous cytosolic and membrane associated enzymes, including phosphatidylinositol 3-OH kinase (PI 3-kinase). Recent reports have suggested that PI 3-kinase may serve as an upstream activator of mitogen-activated protein (MAP) kinase. After stimulation with interleukin-3 and granulocyte-macrophage colony-stimulating factor, we show here that inhibition of MAP kinase activity by two inhibitors of PI 3-kinase, wortmannin and LY-294002, does not correlate with their ability to inhibit PI 3-kinase or p70 S6 kinase phosphorylation. Complete inhibition of phosphatidylinositol 3,4,5-trisphosphate production occurred at approximately 100 nM WM or 25 µM LY-294002, but at these concentrations, WM significantly inhibited MAP kinase activation, while LY-294002 had virtually no effect on MAP kinase activity. Furthermore, WM does not inhibit phorbol ester-mediated MAP kinase activation, but LY-294002 does. Together these results suggest WM and LY-294002 are differentially inhibiting enzymes other than PI 3-kinase that function upstream of MAP kinase." @default.
• W2000653179 created "2016-06-24" @default.
• W2000653179 creator A5050668557 @default.
• W2000653179 creator A5053999665 @default.
• W2000653179 date "1996-07-01" @default.
• W2000653179 modified "2023-09-30" @default.
• W2000653179 title "Phosphatidylinositol 3-OH Kinase Activity Is Not Required for Activation of Mitogen-activated Protein Kinase by Cytokines" @default.
• W2000653179 cites W1505315584 @default.
• W2000653179 cites W1529396499 @default.
• W2000653179 cites W1535997180 @default.
• W2000653179 cites W1552800926 @default.
• W2000653179 cites W1583116012 @default.
• W2000653179 cites W1595612667 @default.
• W2000653179 cites W1613040252 @default.
• W2000653179 cites W172080573 @default.
• W2000653179 cites W1818313483 @default.
• W2000653179 cites W1877370095 @default.
• W2000653179 cites W1947575656 @default.
• W2000653179 cites W1967698763 @default.
• W2000653179 cites W1969161473 @default.
• W2000653179 cites W1974010399 @default.
• W2000653179 cites W1979284770 @default.
• W2000653179 cites W1980403683 @default.
• W2000653179 cites W1986456969 @default.
• W2000653179 cites W1996227816 @default.
• W2000653179 cites W1999012962 @default.
• W2000653179 cites W2019753526 @default.
• W2000653179 cites W2024815029 @default.
• W2000653179 cites W2032013475 @default.
• W2000653179 cites W2050011902 @default.
• W2000653179 cites W2068295905 @default.
• W2000653179 cites W2072876326 @default.
• W2000653179 cites W2077673188 @default.
• W2000653179 cites W2089731973 @default.
• W2000653179 cites W2090051314 @default.
• W2000653179 cites W2098476468 @default.
• W2000653179 cites W2105490477 @default.
• W2000653179 cites W2108707241 @default.
• W2000653179 cites W2123870380 @default.
• W2000653179 cites W2174898638 @default.
• W2000653179 cites W236733732 @default.
• W2000653179 cites W2442275722 @default.
• W2000653179 doi "https://doi.org/10.1074/jbc.271.30.18134" @default.
• W2000653179 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8663437" @default.
• W2000653179 hasPublicationYear "1996" @default.
• W2000653179 type Work @default.
• W2000653179 sameAs 2000653179 @default.
• W2000653179 citedByCount "55" @default.
• W2000653179 countsByYear W20006531792012 @default.
• W2000653179 countsByYear W20006531792013 @default.
• W2000653179 countsByYear W20006531792016 @default.
• W2000653179 crossrefType "journal-article" @default.
• W2000653179 hasAuthorship W2000653179A5050668557 @default.
• W2000653179 hasAuthorship W2000653179A5053999665 @default.
• W2000653179 hasBestOaLocation W20006531791 @default.
• W2000653179 hasConcept C124160383 @default.
• W2000653179 hasConcept C137361374 @default.
• W2000653179 hasConcept C153911025 @default.
• W2000653179 hasConcept C159479382 @default.
• W2000653179 hasConcept C184235292 @default.
• W2000653179 hasConcept C195794163 @default.
• W2000653179 hasConcept C2778308172 @default.
• W2000653179 hasConcept C2780610907 @default.
• W2000653179 hasConcept C55493867 @default.
• W2000653179 hasConcept C59143045 @default.
• W2000653179 hasConcept C82495950 @default.
• W2000653179 hasConcept C86803240 @default.
• W2000653179 hasConcept C90934575 @default.
• W2000653179 hasConcept C95444343 @default.
• W2000653179 hasConcept C97029542 @default.
• W2000653179 hasConceptScore W2000653179C124160383 @default.
• W2000653179 hasConceptScore W2000653179C137361374 @default.
• W2000653179 hasConceptScore W2000653179C153911025 @default.
• W2000653179 hasConceptScore W2000653179C159479382 @default.
• W2000653179 hasConceptScore W2000653179C184235292 @default.
• W2000653179 hasConceptScore W2000653179C195794163 @default.
• W2000653179 hasConceptScore W2000653179C2778308172 @default.
• W2000653179 hasConceptScore W2000653179C2780610907 @default.
• W2000653179 hasConceptScore W2000653179C55493867 @default.
• W2000653179 hasConceptScore W2000653179C59143045 @default.
• W2000653179 hasConceptScore W2000653179C82495950 @default.
• W2000653179 hasConceptScore W2000653179C86803240 @default.
• W2000653179 hasConceptScore W2000653179C90934575 @default.
• W2000653179 hasConceptScore W2000653179C95444343 @default.
• W2000653179 hasConceptScore W2000653179C97029542 @default.
• W2000653179 hasIssue "30" @default.
• W2000653179 hasLocation W20006531791 @default.
• W2000653179 hasOpenAccess W2000653179 @default.
• W2000653179 hasPrimaryLocation W20006531791 @default.
• W2000653179 hasRelatedWork W167860707 @default.
• W2000653179 hasRelatedWork W1967861082 @default.
• W2000653179 hasRelatedWork W1985806168 @default.
• W2000653179 hasRelatedWork W2001173363 @default.
• W2000653179 hasRelatedWork W2013479821 @default.
• W2000653179 hasRelatedWork W2031116438 @default.
• W2000653179 hasRelatedWork W2055437587 @default.
• W2000653179 hasRelatedWork W2056401245 @default.
• W2000653179 hasRelatedWork W2061404590 @default.

• next