FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000654639> ?p ?o ?g. }

• W2000654639 endingPage "378" @default.
• W2000654639 startingPage "359" @default.
• W2000654639 abstract "Nucleotides that neighbor codons in Escherichia coli genes are highly non-random. Furthermore, these context biases are stronger and extend farther from the codon in weakly expressed than in highly expressed genes. We therefore suggested that codon contexts are selected to reduce gene expression levels. We now compare the expression levels of lacZ genes containing two specific coding sequences (context inserts). One context insert represents contexts seen in weakly expressed genes (low variant); the other represents contexts seen in highly expressed genes (high variant). The two variants have identical nucleotide and codon compositions, and encode the same protein. A permutation of four nucleotides, which changes eight codon: codon interfaces of 1043, comprises the only difference between the high and low context variant genes. In three different lacZ mRNAs, the low variant was expressed at a level significantly below that of the high variant. This context effect depends entirely on translation of the contexts in the correct frame; its magnitude depends in part on the placement of other features (e.g. transcriptional pauses and terminators, or perhaps other slow codons or contexts) in the mRNAs. Changing the ribosome density on the message by changing the ribosome binding site distinguishes between dropoff, interference and polarity, three fundamentally different types of models for the context effect. The expression difference between context variants is eliminated by both increases and decreases in the ribosome initiation frequency, as uniquely predicted by the polarity model. In fact, data from all constructions are accommodated by a model in which slow translation of the low context insert increases rho-dependent transcriptional termination within the test gene. The data suggest that the rates of translational initiation and elongation are poised with respect to the rate of transcriptional elongation so that all are influential in setting the expression level of wild-type lacZ. We conclude that context-induced polarity will exist in genes wherever low and reproducible gene product levels have been selected." @default.
• W2000654639 created "2016-06-24" @default.
• W2000654639 creator A5057555892 @default.
• W2000654639 creator A5086295306 @default.
• W2000654639 date "1989-10-01" @default.
• W2000654639 modified "2023-10-05" @default.
• W2000654639 title "Codon contexts from weakly expressed genes reduce expression in vivo" @default.
• W2000654639 cites W1001283980 @default.
• W2000654639 cites W1480722216 @default.
• W2000654639 cites W1486946238 @default.
• W2000654639 cites W1504552716 @default.
• W2000654639 cites W1531492600 @default.
• W2000654639 cites W1555159335 @default.
• W2000654639 cites W1592856823 @default.
• W2000654639 cites W1597915137 @default.
• W2000654639 cites W1599809900 @default.
• W2000654639 cites W1625101491 @default.
• W2000654639 cites W184098300 @default.
• W2000654639 cites W1887049991 @default.
• W2000654639 cites W1965584649 @default.
• W2000654639 cites W1967385588 @default.
• W2000654639 cites W1974025425 @default.
• W2000654639 cites W1974045468 @default.
• W2000654639 cites W1975695704 @default.
• W2000654639 cites W1979109081 @default.
• W2000654639 cites W1981371363 @default.
• W2000654639 cites W1989791847 @default.
• W2000654639 cites W1991480269 @default.
• W2000654639 cites W2002677631 @default.
• W2000654639 cites W2004674004 @default.
• W2000654639 cites W2008566545 @default.
• W2000654639 cites W2008912071 @default.
• W2000654639 cites W2008936546 @default.
• W2000654639 cites W2011696195 @default.
• W2000654639 cites W2017371535 @default.
• W2000654639 cites W2019410656 @default.
• W2000654639 cites W2020302409 @default.
• W2000654639 cites W2021937457 @default.
• W2000654639 cites W2024391369 @default.
• W2000654639 cites W2027059027 @default.
• W2000654639 cites W2027730367 @default.
• W2000654639 cites W2034104563 @default.
• W2000654639 cites W2035499170 @default.
• W2000654639 cites W2040045652 @default.
• W2000654639 cites W2043890113 @default.
• W2000654639 cites W2050077441 @default.
• W2000654639 cites W2052413864 @default.
• W2000654639 cites W2057074984 @default.
• W2000654639 cites W2058418525 @default.
• W2000654639 cites W2062417955 @default.
• W2000654639 cites W2064494607 @default.
• W2000654639 cites W2066464905 @default.
• W2000654639 cites W2067447790 @default.
• W2000654639 cites W2077777080 @default.
• W2000654639 cites W2080054708 @default.
• W2000654639 cites W2084884863 @default.
• W2000654639 cites W2096074458 @default.
• W2000654639 cites W2111390925 @default.
• W2000654639 cites W2123668439 @default.
• W2000654639 cites W2138762386 @default.
• W2000654639 cites W2142336129 @default.
• W2000654639 cites W2144047802 @default.
• W2000654639 cites W2152812151 @default.
• W2000654639 cites W4240480203 @default.
• W2000654639 cites W4245380020 @default.
• W2000654639 doi "https://doi.org/10.1016/0022-2836(89)90003-x" @default.
• W2000654639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2511323" @default.
• W2000654639 hasPublicationYear "1989" @default.
• W2000654639 type Work @default.
• W2000654639 sameAs 2000654639 @default.
• W2000654639 citedByCount "43" @default.
• W2000654639 countsByYear W20006546392012 @default.
• W2000654639 countsByYear W20006546392014 @default.
• W2000654639 countsByYear W20006546392016 @default.
• W2000654639 countsByYear W20006546392018 @default.
• W2000654639 countsByYear W20006546392020 @default.
• W2000654639 crossrefType "journal-article" @default.
• W2000654639 hasAuthorship W2000654639A5057555892 @default.
• W2000654639 hasAuthorship W2000654639A5086295306 @default.
• W2000654639 hasConcept C104317684 @default.
• W2000654639 hasConcept C105580179 @default.
• W2000654639 hasConcept C114847915 @default.
• W2000654639 hasConcept C141231307 @default.
• W2000654639 hasConcept C149364088 @default.
• W2000654639 hasConcept C150194340 @default.
• W2000654639 hasConcept C151730666 @default.
• W2000654639 hasConcept C153957851 @default.
• W2000654639 hasConcept C167625842 @default.
• W2000654639 hasConcept C176990463 @default.
• W2000654639 hasConcept C20580545 @default.
• W2000654639 hasConcept C2779343474 @default.
• W2000654639 hasConcept C47289529 @default.
• W2000654639 hasConcept C54355233 @default.
• W2000654639 hasConcept C67705224 @default.
• W2000654639 hasConcept C86803240 @default.
• W2000654639 hasConcept C87253356 @default.
• W2000654639 hasConcept C88478588 @default.
• W2000654639 hasConcept C91779695 @default.

• next