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- W2000654960 abstract "A water-soluble, (1→6)-branched, (1→3)-β-d-glucan (T-4-N), [α]D20 +19° (c 0.1, water), was isolated from a 2% sodium carbonate extract of the fruit bodies of Dictyophora indusiata Fisch. T-4-N was homogeneous as judged by gel filtration, Tiselius-type electrophoresis, and ultracentrifugal analysis. By gel filtration on Sepharose CL-2B, with 0.25m sodium hydroxide as the eluant, the molecular weight of T-4-N was estimated to be ∼5.5 × 106. From the results of methylation analysis, periodate oxidation, Smith degradation (complete, and also mild), partial acetolysis, and enzymic degradation, it was concluded that T-4-N has a main chain composed of β-(1→3)-linked d-glucopyranosyl residues, and two single, β-(1→6)-linked d-glucopyranosyl groups attached as side chains to, on average, every fifth sugar residue of the main chain. In addition, the results of the enzymic hydrolysis suggested that the β-(1→6)-linked d-glucosyl side chains are mainly localized in the neighborhood of the nonreducing end of the main chain. The results of optical rotatory measurement and complex-formation with Congo Red indicated that T-4-N probably takes a triple-helical conformation." @default.
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- W2000654960 date "1983-06-01" @default.
- W2000654960 modified "2023-09-24" @default.
- W2000654960 title "A branched (1→3)-β-d-glucan from a sodium carbonate extract of Dictyophora indusiata fisch" @default.
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- W2000654960 doi "https://doi.org/10.1016/0008-6215(83)88087-2" @default.
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