Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000656127> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2000656127 endingPage "83" @default.
- W2000656127 startingPage "71" @default.
- W2000656127 abstract "Immunoglobulin fragments, whether of polyclonal or monoclonal antibodies, offer a number of advantages over the intact immunoglobulin. The generation of immunoreactive fragments from monoclonal antibodies (MAb) is not always a straightforward task. Both pepsin and papain can be used to digest MAb to a bivalent molecule with a Mr of 100 000. However, pepsin digestion does not always result in immunoreactive fragments and a stable consistent product by papain digestion is often difficult to obtain. MAb B72.3 is an example of both situations. MAb B72.3 reacts with a glycoprotein (TAG-72) with a molecular weight greater than 106. MAb B72.3 has been shown to exhibit a high degree of selective reactivity with colon, breast and ovarian carcinomas and has been used for radioimmunodiagnosis in model systems and in clinical trials. A third enzyme, bromelain, in the same family of sulfhydryl proteases as papain, has been used to generate a fragment of MAb B72.3, with a Mr of approximately 100 000. The bromelain-generated fragment of MAb B72.3 retained 100% immunoreactivity as measured in competitive solid-phase radioimmunoassays and could be generated with consistent results from one preparation to another. Both the bromelain- and papain-generated fragments were radiolabelled with 125I without significant loss of the MAb's reactivity to tumor extracts. Differences were observed between the bromelain- and papain-generated fragment when compared in vivo. Fragmentation of MAb B72.3 with bromelain has yielded a superior bivalent fragment for radioimmunolocalization." @default.
- W2000656127 created "2016-06-24" @default.
- W2000656127 creator A5066817014 @default.
- W2000656127 creator A5082864167 @default.
- W2000656127 creator A5088599670 @default.
- W2000656127 date "1989-06-01" @default.
- W2000656127 modified "2023-09-24" @default.
- W2000656127 title "Comparison of methods for the generation of immunoreactive fragments of a monoclonal antibody (B72.3) reactive with human carcinomas" @default.
- W2000656127 cites W136904606 @default.
- W2000656127 cites W1498873358 @default.
- W2000656127 cites W1533725999 @default.
- W2000656127 cites W1775749144 @default.
- W2000656127 cites W1786523947 @default.
- W2000656127 cites W1965512850 @default.
- W2000656127 cites W1974242613 @default.
- W2000656127 cites W1976597045 @default.
- W2000656127 cites W2005025930 @default.
- W2000656127 cites W2026993269 @default.
- W2000656127 cites W2034413378 @default.
- W2000656127 cites W2046907336 @default.
- W2000656127 cites W2080580789 @default.
- W2000656127 cites W2084168547 @default.
- W2000656127 cites W2086341520 @default.
- W2000656127 cites W2089120635 @default.
- W2000656127 cites W2096668339 @default.
- W2000656127 cites W2097874416 @default.
- W2000656127 cites W2100837269 @default.
- W2000656127 cites W2120977313 @default.
- W2000656127 cites W2145011046 @default.
- W2000656127 cites W2176780488 @default.
- W2000656127 doi "https://doi.org/10.1016/0022-1759(89)90291-3" @default.
- W2000656127 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2499638" @default.
- W2000656127 hasPublicationYear "1989" @default.
- W2000656127 type Work @default.
- W2000656127 sameAs 2000656127 @default.
- W2000656127 citedByCount "56" @default.
- W2000656127 countsByYear W20006561272013 @default.
- W2000656127 countsByYear W20006561272014 @default.
- W2000656127 countsByYear W20006561272015 @default.
- W2000656127 countsByYear W20006561272016 @default.
- W2000656127 countsByYear W20006561272018 @default.
- W2000656127 countsByYear W20006561272022 @default.
- W2000656127 crossrefType "journal-article" @default.
- W2000656127 hasAuthorship W2000656127A5066817014 @default.
- W2000656127 hasAuthorship W2000656127A5082864167 @default.
- W2000656127 hasAuthorship W2000656127A5088599670 @default.
- W2000656127 hasConcept C108625454 @default.
- W2000656127 hasConcept C153911025 @default.
- W2000656127 hasConcept C159654299 @default.
- W2000656127 hasConcept C163864487 @default.
- W2000656127 hasConcept C181199279 @default.
- W2000656127 hasConcept C185592680 @default.
- W2000656127 hasConcept C203014093 @default.
- W2000656127 hasConcept C2777334355 @default.
- W2000656127 hasConcept C2779832904 @default.
- W2000656127 hasConcept C542903549 @default.
- W2000656127 hasConcept C55493867 @default.
- W2000656127 hasConcept C86803240 @default.
- W2000656127 hasConcept C956191 @default.
- W2000656127 hasConceptScore W2000656127C108625454 @default.
- W2000656127 hasConceptScore W2000656127C153911025 @default.
- W2000656127 hasConceptScore W2000656127C159654299 @default.
- W2000656127 hasConceptScore W2000656127C163864487 @default.
- W2000656127 hasConceptScore W2000656127C181199279 @default.
- W2000656127 hasConceptScore W2000656127C185592680 @default.
- W2000656127 hasConceptScore W2000656127C203014093 @default.
- W2000656127 hasConceptScore W2000656127C2777334355 @default.
- W2000656127 hasConceptScore W2000656127C2779832904 @default.
- W2000656127 hasConceptScore W2000656127C542903549 @default.
- W2000656127 hasConceptScore W2000656127C55493867 @default.
- W2000656127 hasConceptScore W2000656127C86803240 @default.
- W2000656127 hasConceptScore W2000656127C956191 @default.
- W2000656127 hasIssue "1" @default.
- W2000656127 hasLocation W20006561271 @default.
- W2000656127 hasLocation W20006561272 @default.
- W2000656127 hasOpenAccess W2000656127 @default.
- W2000656127 hasPrimaryLocation W20006561271 @default.
- W2000656127 hasRelatedWork W2000656127 @default.
- W2000656127 hasRelatedWork W2020465807 @default.
- W2000656127 hasRelatedWork W2025678534 @default.
- W2000656127 hasRelatedWork W2056973121 @default.
- W2000656127 hasRelatedWork W2073840950 @default.
- W2000656127 hasRelatedWork W2146940419 @default.
- W2000656127 hasRelatedWork W2366047017 @default.
- W2000656127 hasRelatedWork W2370919143 @default.
- W2000656127 hasRelatedWork W2418580403 @default.
- W2000656127 hasRelatedWork W2096939959 @default.
- W2000656127 hasVolume "120" @default.
- W2000656127 isParatext "false" @default.
- W2000656127 isRetracted "false" @default.
- W2000656127 magId "2000656127" @default.
- W2000656127 workType "article" @default.