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• W2000657631 abstract "1 Growth of baby hamster kidney (BHK) cells in medium containing 2-deoxy-d-glucose is retarded in direct proportion to the 2-deoxyglucose concentration. The severity of the effect is reduced in medium containing high relative concentrations of glucose. 2 2-Deoxyglucose inhibits the incorporation of radioactivity from mannose, galactose, glucosamine, fucose and N-acetylmannosamine precursors into acid-insoluble cellular material. Incorporation of radioactively labelled leucine into protein is not affected by 2-deoxyglucose. 3 BHK cells grown in the presence of 2-deoxyglucose become less sensitive to the toxic action of certain plant lectins, ricin of Ricinus communis and Phaseolus vulgaris phytohaemagglutinin, which bind specifically to cell surface galactose and N-acetyl-galactosamine residues. By contrast, 2-deoxyglucose increased the sensitivity of BHK cells to the weak toxicity of concanavalin A which binds to surface mannosides. Treated cells also become more agglutinable with concanavalin A. 4 Cell surface glycoproteins labelled by lactoperoxidase-catalysed iodination have been examined by dodecylsulphate-polyacrylamide gel electrophoresis. The radio-iodinated glycoproteins prepared from cells grown in medium containing 2-deoxyglucose migrate more rapidly than glycoproteins from cells grown in the absence of inhibitor." @default.
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• W2000657631 date "1977-01-01" @default.
• W2000657631 modified "2023-10-16" @default.
• W2000657631 title "Effect of 2-Deoxy-d-glucose on the Cell-Surface Glycoproteins of Hamster Fibroblasts" @default.
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• W2000657631 doi "https://doi.org/10.1111/j.1432-1033.1977.tb11249.x" @default.
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