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- W2000660038 abstract "A two-stage culture strategy was studied for continuous high-level production of a foreign protein in the chemically inducible T7 expression system. The first stage is dedicated to the maintenance of plasmid-bearing cells and the second stage to the target protein synthesis by induction of cells coming from the first stage. On entering the second stage, recombinant cells undergo a gradual induction of the target gene expression. These plasmid-bearing cells experience dynamic changes in intracellular compositions and specific growth rates with their individual residence times. Therefore, the overall cultural characteristics in the production stage are really averages of the contributions from the various cells with different residence times. The behavior of the two-stage culture is described by a model, which accounts for dynamic variations of cell growth and protein synthesis rates with cell residence times. Model simulations were compared with experimental results at a variety of operating conditions such as inducer concentration and dilution rate. This model is useful for understanding the behavior of two-stage continuous cultures." @default.
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- W2000660038 date "1993-06-05" @default.
- W2000660038 modified "2023-09-24" @default.
- W2000660038 title "Overexpression of cloned genes using recombinantescherichia coli regulated by a T7 promoter: II. Two-stage continuous cultures and model simulations" @default.
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- W2000660038 doi "https://doi.org/10.1002/bit.260420110" @default.
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