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- W2000664893 abstract "African green monkey cells (CV-1P) were microinjected with highly purified SV40 T antigen using protein-loaded red cell ghosts and polyethylene glycol as fusagen. The microinjected cells were infected with a temperature-sensitive mutant of SV40 (tsA209) which is defective in the initiation of viral DNA synthesis. Using in situ hybridization as an assay method, we found that PEG-microinjection of both partially and highly purified T antigen resulted in an increase in the amount of viral DNA sequences in the monolayer. Moreover, 3H-thymidine-labeled and unlabeled Hirt supernatant from microinjected, tsA209-injected cells contained significantly more SV40 DNA than comparable extracts from sham-injected, tsA209-infected or uninfected cells, which were tested in parallel. Thus the introduction of highly purified, large SV40 T antigen led to phenotypic complementation of the tsA defect in viral DNA synthesis." @default.
- W2000664893 created "2016-06-24" @default.
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- W2000664893 date "1978-08-01" @default.
- W2000664893 modified "2023-09-24" @default.
- W2000664893 title "Phenotypic complementation of the SV40 tsA mutant defect in viral DNA synthesis following microinjection of SV40 T antigen" @default.
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- W2000664893 doi "https://doi.org/10.1016/0092-8674(78)90352-5" @default.
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