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• W2000676188 abstract "Knowledge of cardiomyocyte biology is limited by the lack of methods to interrogate single-cell physiology in vivo. Here we show that contracting myocytes can indeed be imaged with optical microscopy at high temporal and spatial resolution in the beating murine heart, allowing visualization of individual sarcomeres and measurement of the single cardiomyocyte contractile cycle. Collectively, this has been enabled by efficient tissue stabilization, a prospective real-time cardiac gating approach, an image processing algorithm for motion-artifact-free imaging throughout the cardiac cycle, and a fluorescent membrane staining protocol. Quantification of cardiomyocyte contractile function in vivo opens many possibilities for investigating myocardial disease and therapeutic intervention at the cellular level." @default.
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• W2000676188 date "2014-07-22" @default.
• W2000676188 modified "2023-10-09" @default.
• W2000676188 title "Intravital imaging of cardiac function at the single-cell level" @default.
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• W2000676188 doi "https://doi.org/10.1073/pnas.1401316111" @default.
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