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- W2000679560 abstract "α-Hydroxyglutarate dependent NADH oxidizing activity was found in a cell-free extract of Alcaligenes No. 314. Succinate was identified as a product from L-α-hydroxyglutarate in the extract. Stoichiometric conversion of L-α-hydroxyglutarate to succinate occurred under anaerobic conditions without the addition of any cofactors, while the reaction was stimulated by Fe2+. 4,5-Dioxovaleric acid seems to be an intermediate of the reaction since it was converted to succinate and L-α-hydroxyglutarate by the extract. Addition of formaldehyde, another possible product of the succinate forming reaction, to the extract caused oxidation of NADH, which might be responsible for the α-hydroxyglutarate dependent NADH oxidation. Considering the presence of L-α-hydroxyglutarate dehydrogenase in the Alcaligenes cells, an anaerobic by-path of the TCA cycle through which succinate is formed from α-ketoglutarate was proposed." @default.
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- W2000679560 date "1986-01-01" @default.
- W2000679560 modified "2023-09-25" @default.
- W2000679560 title "Metabolism of .ALPHA.-hydroxyglutarate by an Alcaligenes sp." @default.
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- W2000679560 doi "https://doi.org/10.1271/bbb1961.50.1145" @default.
- W2000679560 hasPublicationYear "1986" @default.
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