FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000683840> ?p ?o ?g. }

• W2000683840 endingPage "1350" @default.
• W2000683840 startingPage "1343" @default.
• W2000683840 abstract "Angiogenin (ANG), a homologue of bovine pancreatic ribonuclease A (RNase A), promotes the growth of new blood vessels. The biological activity of ANG is dependent on its ribonucleolytic activity, which is far lower than that of RNase A. Here, the efficient heterologous production of human ANG in Escherichia coli was achieved by replacing two sequences of rare codons with codons favored by E. coli. Hypersensitive fluorogenic substrates were used to determine steady-state kinetic parameters for catalysis by ANG in continuous assays. The ANG pH-rate profile is a classic bell-shaped curve, with pK(1) = 5.0 and pK(2) = 7.0. The ribonucleolytic activity of ANG is highly sensitive to Na(+) concentration. A decrease in Na(+) concentration from 0.25 to 0.025 M causes a 170-fold increase in the value of k(cat)/K(M). Likewise, the binding of ANG to a tetranucleotide substrate analogue is dependent on [Na(+)]. ANG cleaves a dinucleotide version of the fluorogenic substrates with a k(cat)/K(M) value of 61 M(-1) s(-1). When the substrate is extended from two nucleotides to four or six nucleotides, values of k(cat)/K(M) increase by 5- and 12-fold, respectively. Together, these data provide a thorough picture of substrate binding and turnover by ANG." @default.
• W2000683840 created "2016-06-24" @default.
• W2000683840 creator A5004483777 @default.
• W2000683840 creator A5019213891 @default.
• W2000683840 creator A5035811227 @default.
• W2000683840 creator A5086857855 @default.
• W2000683840 creator A5091197853 @default.
• W2000683840 date "2002-01-01" @default.
• W2000683840 modified "2023-09-27" @default.
• W2000683840 title "The Ribonucleolytic Activity of Angiogenin" @default.
• W2000683840 cites W1799140735 @default.
• W2000683840 cites W1968966912 @default.
• W2000683840 cites W1975872856 @default.
• W2000683840 cites W1987797744 @default.
• W2000683840 cites W2000000541 @default.
• W2000683840 cites W2009447624 @default.
• W2000683840 cites W2022788699 @default.
• W2000683840 cites W2030836217 @default.
• W2000683840 cites W2054728198 @default.
• W2000683840 cites W2079463373 @default.
• W2000683840 cites W2093789100 @default.
• W2000683840 cites W2094088429 @default.
• W2000683840 cites W2118839351 @default.
• W2000683840 cites W2197678796 @default.
• W2000683840 cites W221915106 @default.
• W2000683840 cites W4211216439 @default.
• W2000683840 cites W4245635197 @default.
• W2000683840 doi "https://doi.org/10.1021/bi0117899" @default.
• W2000683840 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11802736" @default.
• W2000683840 hasPublicationYear "2002" @default.
• W2000683840 type Work @default.
• W2000683840 sameAs 2000683840 @default.
• W2000683840 citedByCount "49" @default.
• W2000683840 countsByYear W20006838402012 @default.
• W2000683840 countsByYear W20006838402013 @default.
• W2000683840 countsByYear W20006838402015 @default.
• W2000683840 countsByYear W20006838402016 @default.
• W2000683840 countsByYear W20006838402017 @default.
• W2000683840 countsByYear W20006838402018 @default.
• W2000683840 countsByYear W20006838402019 @default.
• W2000683840 countsByYear W20006838402020 @default.
• W2000683840 countsByYear W20006838402021 @default.
• W2000683840 countsByYear W20006838402022 @default.
• W2000683840 countsByYear W20006838402023 @default.
• W2000683840 crossrefType "journal-article" @default.
• W2000683840 hasAuthorship W2000683840A5004483777 @default.
• W2000683840 hasAuthorship W2000683840A5019213891 @default.
• W2000683840 hasAuthorship W2000683840A5035811227 @default.
• W2000683840 hasAuthorship W2000683840A5086857855 @default.
• W2000683840 hasAuthorship W2000683840A5091197853 @default.
• W2000683840 hasConcept C104317684 @default.
• W2000683840 hasConcept C10879258 @default.
• W2000683840 hasConcept C153911025 @default.
• W2000683840 hasConcept C185592680 @default.
• W2000683840 hasConcept C18903297 @default.
• W2000683840 hasConcept C2776719180 @default.
• W2000683840 hasConcept C2776813977 @default.
• W2000683840 hasConcept C2777289219 @default.
• W2000683840 hasConcept C2778976237 @default.
• W2000683840 hasConcept C2780115692 @default.
• W2000683840 hasConcept C2780394083 @default.
• W2000683840 hasConcept C2780704907 @default.
• W2000683840 hasConcept C502942594 @default.
• W2000683840 hasConcept C512185932 @default.
• W2000683840 hasConcept C547475151 @default.
• W2000683840 hasConcept C55493867 @default.
• W2000683840 hasConcept C67705224 @default.
• W2000683840 hasConcept C86803240 @default.
• W2000683840 hasConceptScore W2000683840C104317684 @default.
• W2000683840 hasConceptScore W2000683840C10879258 @default.
• W2000683840 hasConceptScore W2000683840C153911025 @default.
• W2000683840 hasConceptScore W2000683840C185592680 @default.
• W2000683840 hasConceptScore W2000683840C18903297 @default.
• W2000683840 hasConceptScore W2000683840C2776719180 @default.
• W2000683840 hasConceptScore W2000683840C2776813977 @default.
• W2000683840 hasConceptScore W2000683840C2777289219 @default.
• W2000683840 hasConceptScore W2000683840C2778976237 @default.
• W2000683840 hasConceptScore W2000683840C2780115692 @default.
• W2000683840 hasConceptScore W2000683840C2780394083 @default.
• W2000683840 hasConceptScore W2000683840C2780704907 @default.
• W2000683840 hasConceptScore W2000683840C502942594 @default.
• W2000683840 hasConceptScore W2000683840C512185932 @default.
• W2000683840 hasConceptScore W2000683840C547475151 @default.
• W2000683840 hasConceptScore W2000683840C55493867 @default.
• W2000683840 hasConceptScore W2000683840C67705224 @default.
• W2000683840 hasConceptScore W2000683840C86803240 @default.
• W2000683840 hasIssue "4" @default.
• W2000683840 hasLocation W20006838401 @default.
• W2000683840 hasLocation W20006838402 @default.
• W2000683840 hasOpenAccess W2000683840 @default.
• W2000683840 hasPrimaryLocation W20006838401 @default.
• W2000683840 hasRelatedWork W150281572 @default.
• W2000683840 hasRelatedWork W190386510 @default.
• W2000683840 hasRelatedWork W1967129754 @default.
• W2000683840 hasRelatedWork W1985406337 @default.
• W2000683840 hasRelatedWork W2000683840 @default.
• W2000683840 hasRelatedWork W200781673 @default.
• W2000683840 hasRelatedWork W2014737439 @default.

• next