FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000689693> ?p ?o ?g. }

• W2000689693 endingPage "19" @default.
• W2000689693 startingPage "10" @default.
• W2000689693 abstract "Leukemia inhibitory factor (LIF) is a pleiotropic cytokine mainly produced by activated T lymphocytes. We previously demonstrated that human Jurkat T lymphoma cells represent a valid model of LIF gene expression. This study was designed to identify regions critical for LIF promoter activation in Jurkat cells.Luciferase constructs under the control of different portions of the human LIF promoter were transfected into Jurkat cells, and promoter activity was determined by luminometry. Similar experiments were performed with constructs bearing mutations in the putative ETS binding regions in the LIF promoter. RT-PCR, Western blot and gelshift experiments were performed to study expression and DNA binding of ETS factors in lymphoid cells.With the exception of the shortest construct not including the putative ETS binding sites, all wildtype LIF promoter constructs were strongly inducible by phorbol ester/ionomycin. In contrast, the mutant constructs were significantly less inducible. Cotransfection of the wild-type constructs with ETS expression vectors resulted in significant enhancement of promoter activity. ets-1 and ets-2 mRNA and protein were shown to be expressed in Jurkat cells. Gelshift experiments revealed that proteins present in nuclear extracts from Jurkat cells specifically bind to both artificial ETS consensus sites and ETS binding sites present in the LIF promoter.We conclude that binding of ETS transcription factors to the ETS binding sites in the human LIF promoter is critical for its inducibility in response to T cell activators. ETS transcription factors thus play an important functional role within the endocrine-immune network." @default.
• W2000689693 created "2016-06-24" @default.
• W2000689693 creator A5015749578 @default.
• W2000689693 creator A5017106218 @default.
• W2000689693 creator A5032742966 @default.
• W2000689693 creator A5045797580 @default.
• W2000689693 creator A5048396622 @default.
• W2000689693 creator A5078720772 @default.
• W2000689693 date "2003-10-17" @default.
• W2000689693 modified "2023-10-13" @default.
• W2000689693 title "Regulation of the Human Leukemia Inhibitory Factor Gene by <i>ets</i> Transcription Factors" @default.
• W2000689693 cites W1586382415 @default.
• W2000689693 cites W1964279670 @default.
• W2000689693 cites W1967979656 @default.
• W2000689693 cites W1971068712 @default.
• W2000689693 cites W1980692466 @default.
• W2000689693 cites W1991862686 @default.
• W2000689693 cites W1998973642 @default.
• W2000689693 cites W2007559737 @default.
• W2000689693 cites W2010428440 @default.
• W2000689693 cites W2037666513 @default.
• W2000689693 cites W2039081691 @default.
• W2000689693 cites W2040578136 @default.
• W2000689693 cites W2056416376 @default.
• W2000689693 cites W2057494454 @default.
• W2000689693 cites W2076340895 @default.
• W2000689693 cites W2110670037 @default.
• W2000689693 cites W2142446767 @default.
• W2000689693 cites W2151778247 @default.
• W2000689693 cites W2164931869 @default.
• W2000689693 cites W2167132627 @default.
• W2000689693 cites W2328599562 @default.
• W2000689693 cites W4233006284 @default.
• W2000689693 cites W4235095653 @default.
• W2000689693 doi "https://doi.org/10.1159/000072964" @default.
• W2000689693 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14557674" @default.
• W2000689693 hasPublicationYear "2003" @default.
• W2000689693 type Work @default.
• W2000689693 sameAs 2000689693 @default.
• W2000689693 citedByCount "13" @default.
• W2000689693 countsByYear W20006896932013 @default.
• W2000689693 countsByYear W20006896932015 @default.
• W2000689693 countsByYear W20006896932019 @default.
• W2000689693 countsByYear W20006896932022 @default.
• W2000689693 crossrefType "journal-article" @default.
• W2000689693 hasAuthorship W2000689693A5015749578 @default.
• W2000689693 hasAuthorship W2000689693A5017106218 @default.
• W2000689693 hasAuthorship W2000689693A5032742966 @default.
• W2000689693 hasAuthorship W2000689693A5045797580 @default.
• W2000689693 hasAuthorship W2000689693A5048396622 @default.
• W2000689693 hasAuthorship W2000689693A5078720772 @default.
• W2000689693 hasConcept C101762097 @default.
• W2000689693 hasConcept C104317684 @default.
• W2000689693 hasConcept C132376346 @default.
• W2000689693 hasConcept C145103041 @default.
• W2000689693 hasConcept C150194340 @default.
• W2000689693 hasConcept C153911025 @default.
• W2000689693 hasConcept C165864922 @default.
• W2000689693 hasConcept C179464577 @default.
• W2000689693 hasConcept C27284151 @default.
• W2000689693 hasConcept C2776090121 @default.
• W2000689693 hasConcept C54009773 @default.
• W2000689693 hasConcept C54355233 @default.
• W2000689693 hasConcept C86339819 @default.
• W2000689693 hasConcept C86803240 @default.
• W2000689693 hasConcept C8891405 @default.
• W2000689693 hasConceptScore W2000689693C101762097 @default.
• W2000689693 hasConceptScore W2000689693C104317684 @default.
• W2000689693 hasConceptScore W2000689693C132376346 @default.
• W2000689693 hasConceptScore W2000689693C145103041 @default.
• W2000689693 hasConceptScore W2000689693C150194340 @default.
• W2000689693 hasConceptScore W2000689693C153911025 @default.
• W2000689693 hasConceptScore W2000689693C165864922 @default.
• W2000689693 hasConceptScore W2000689693C179464577 @default.
• W2000689693 hasConceptScore W2000689693C27284151 @default.
• W2000689693 hasConceptScore W2000689693C2776090121 @default.
• W2000689693 hasConceptScore W2000689693C54009773 @default.
• W2000689693 hasConceptScore W2000689693C54355233 @default.
• W2000689693 hasConceptScore W2000689693C86339819 @default.
• W2000689693 hasConceptScore W2000689693C86803240 @default.
• W2000689693 hasConceptScore W2000689693C8891405 @default.
• W2000689693 hasIssue "1" @default.
• W2000689693 hasLocation W20006896931 @default.
• W2000689693 hasLocation W20006896932 @default.
• W2000689693 hasOpenAccess W2000689693 @default.
• W2000689693 hasPrimaryLocation W20006896931 @default.
• W2000689693 hasRelatedWork W1988402913 @default.
• W2000689693 hasRelatedWork W2046386509 @default.
• W2000689693 hasRelatedWork W2059358614 @default.
• W2000689693 hasRelatedWork W2075341877 @default.
• W2000689693 hasRelatedWork W2148922163 @default.
• W2000689693 hasRelatedWork W2218604244 @default.
• W2000689693 hasRelatedWork W2382526585 @default.
• W2000689693 hasRelatedWork W2581702729 @default.
• W2000689693 hasRelatedWork W2786667712 @default.
• W2000689693 hasRelatedWork W2896653760 @default.
• W2000689693 hasVolume "11" @default.
• W2000689693 isParatext "false" @default.

• next