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- W2000693703 endingPage "465" @default.
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- W2000693703 abstract "A fusion tag, called FLAG and consisting of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions [Bio/Technology, 6 (1988) 1204]. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAG peptide. Antibodies M2 and M5 are applied in this procedure. Examples demonstrating the versatility, practicability and limitations of this technology are given." @default.
- W2000693703 created "2016-06-24" @default.
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- W2000693703 date "2001-10-01" @default.
- W2000693703 modified "2023-10-10" @default.
- W2000693703 title "The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins" @default.
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- W2000693703 doi "https://doi.org/10.1016/s0165-022x(01)00213-5" @default.
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