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- W2000694802 abstract "Atomic force microscopy (AFM) enables high-resolution three-dimensional (3D) imaging of cultured bone marrow-derived mast cells. Cells were immobilized by a quick centrifugation and fixation to preserve their transient cellular morphologies followed by AFM characterization in buffer. This fix-and-look approach preserves the structural integrity of individual cells. Well-known membrane morphologies, such as ridges and microvilli, are visualized, consistent with prior electron microscopy observations. Additional information including the 3D measurements of these characteristic features are attained from AFM topographs. Filopodia and lamellopodia, associated with cell spreading, were captured and visualized in three dimensions. New morphologies are also revealed, such as high-density ridges and micro-craters. This investigation demonstrates that the fix-and-look approach followed by AFM imaging provides an effective means to characterize the membrane structure of hydrated cells with high resolution. The quantitative imaging and measurements pave the way for systematic correlation of membrane structural features with the biological status of individual cells." @default.
- W2000694802 created "2016-06-24" @default.
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- W2000694802 date "2008-12-01" @default.
- W2000694802 modified "2023-09-27" @default.
- W2000694802 title "High-resolution three-dimensional imaging of the rich membrane structures of bone marrow-derived mast cells" @default.
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- W2000694802 doi "https://doi.org/10.1016/j.ultramic.2008.07.007" @default.
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