FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000696957> ?p ?o ?g. }

• W2000696957 endingPage "121" @default.
• W2000696957 startingPage "112" @default.
• W2000696957 abstract "The BAK1-interacting receptor-like kinase 2 (BIR2) belongs to the large family of leucine-rich repeat receptor-like kinases (LRR-RLKs) that mediate development and innate immunity in plants and form a monophyletic gene family with the Drosophila Pelle and human interleukin-1 receptor-associated kinases (IRAK). BIR2 is a negative regulator of BAK1-mediated defense mechanisms and cell death responses, yet key residues that are typically required for kinase activity are not present in the BIR2 kinase domain. We have determined the crystal structure of the BIR2 cytosolic domain and show that its nucleotide binding site is occluded. NMR spectroscopy confirmed that neither wild type nor phosphorylation-mimicking mutants of BIR2 bind ATP-analogues in solution, suggesting that BIR2 is a genuine enzymatically inactive pseudokinase. BIR2 is, however, phosphorylated by its target of regulation, BAK1. Using nano LC–MS/MS analysis for site-specific analysis of phosphorylation, we found a high density of BAK1-transphosphorylation sites in the BIR2 juxta membrane domain, a region previously implicated in regulation of RLKs. Our findings provide a structural basis to better understand signaling through kinase-dead domains that are predicted to account for 20% of all Arabidopsis RLKs and 10% of all human kinases." @default.
• W2000696957 created "2016-06-24" @default.
• W2000696957 creator A5004225555 @default.
• W2000696957 creator A5010518596 @default.
• W2000696957 creator A5016198415 @default.
• W2000696957 creator A5017978933 @default.
• W2000696957 creator A5029265500 @default.
• W2000696957 creator A5070072027 @default.
• W2000696957 creator A5079814195 @default.
• W2000696957 date "2014-04-01" @default.
• W2000696957 modified "2023-10-16" @default.
• W2000696957 title "Structure of the pseudokinase domain of BIR2, a regulator of BAK1-mediated immune signaling in Arabidopsis" @default.
• W2000696957 cites W104024537 @default.
• W2000696957 cites W1966969980 @default.
• W2000696957 cites W1977922844 @default.
• W2000696957 cites W1978179863 @default.
• W2000696957 cites W1978840865 @default.
• W2000696957 cites W1979178971 @default.
• W2000696957 cites W1982252718 @default.
• W2000696957 cites W2000350495 @default.
• W2000696957 cites W2001641653 @default.
• W2000696957 cites W2008708467 @default.
• W2000696957 cites W2016765195 @default.
• W2000696957 cites W2016882798 @default.
• W2000696957 cites W2017507144 @default.
• W2000696957 cites W2024443524 @default.
• W2000696957 cites W2024873935 @default.
• W2000696957 cites W2030205108 @default.
• W2000696957 cites W2030517878 @default.
• W2000696957 cites W2035442355 @default.
• W2000696957 cites W2035503835 @default.
• W2000696957 cites W2038840577 @default.
• W2000696957 cites W2043216274 @default.
• W2000696957 cites W2052002049 @default.
• W2000696957 cites W2059744667 @default.
• W2000696957 cites W2077276598 @default.
• W2000696957 cites W2079156404 @default.
• W2000696957 cites W2080752012 @default.
• W2000696957 cites W2083786962 @default.
• W2000696957 cites W2089361961 @default.
• W2000696957 cites W2091831292 @default.
• W2000696957 cites W2093889569 @default.
• W2000696957 cites W2099347422 @default.
• W2000696957 cites W2099929909 @default.
• W2000696957 cites W2104408271 @default.
• W2000696957 cites W2105702722 @default.
• W2000696957 cites W2106232004 @default.
• W2000696957 cites W2108959691 @default.
• W2000696957 cites W2110216491 @default.
• W2000696957 cites W2113835577 @default.
• W2000696957 cites W2115460333 @default.
• W2000696957 cites W2117217503 @default.
• W2000696957 cites W2120408260 @default.
• W2000696957 cites W2120772351 @default.
• W2000696957 cites W2126103104 @default.
• W2000696957 cites W2127192155 @default.
• W2000696957 cites W2131639373 @default.
• W2000696957 cites W2132334410 @default.
• W2000696957 cites W2132801043 @default.
• W2000696957 cites W2141150979 @default.
• W2000696957 cites W2141986488 @default.
• W2000696957 cites W2144081223 @default.
• W2000696957 cites W2144362290 @default.
• W2000696957 cites W2147874841 @default.
• W2000696957 cites W2156480395 @default.
• W2000696957 cites W2159731150 @default.
• W2000696957 cites W2163085584 @default.
• W2000696957 cites W2168810887 @default.
• W2000696957 cites W2170141910 @default.
• W2000696957 cites W2180229411 @default.
• W2000696957 cites W4248219898 @default.
• W2000696957 cites W4296928319 @default.
• W2000696957 doi "https://doi.org/10.1016/j.jsb.2014.02.005" @default.
• W2000696957 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24556575" @default.
• W2000696957 hasPublicationYear "2014" @default.
• W2000696957 type Work @default.
• W2000696957 sameAs 2000696957 @default.
• W2000696957 citedByCount "52" @default.
• W2000696957 countsByYear W20006969572014 @default.
• W2000696957 countsByYear W20006969572015 @default.
• W2000696957 countsByYear W20006969572016 @default.
• W2000696957 countsByYear W20006969572017 @default.
• W2000696957 countsByYear W20006969572018 @default.
• W2000696957 countsByYear W20006969572019 @default.
• W2000696957 countsByYear W20006969572020 @default.
• W2000696957 countsByYear W20006969572021 @default.
• W2000696957 countsByYear W20006969572022 @default.
• W2000696957 countsByYear W20006969572023 @default.
• W2000696957 crossrefType "journal-article" @default.
• W2000696957 hasAuthorship W2000696957A5004225555 @default.
• W2000696957 hasAuthorship W2000696957A5010518596 @default.
• W2000696957 hasAuthorship W2000696957A5016198415 @default.
• W2000696957 hasAuthorship W2000696957A5017978933 @default.
• W2000696957 hasAuthorship W2000696957A5029265500 @default.
• W2000696957 hasAuthorship W2000696957A5070072027 @default.
• W2000696957 hasAuthorship W2000696957A5079814195 @default.
• W2000696957 hasConcept C104317684 @default.
• W2000696957 hasConcept C11960822 @default.

• next