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- W2000705460 abstract "Staphylococcus aureus P83 has Panton-Valentine leukocidin (PVL)-like genes, lukM and lukF-PV. Here, lukM and lukF-PV genes were found on the genome of a prophage, which was designated as phiPV83-pro. The precise genome size was 45,636 bp with att core sequences of 10 base pairs. Sixty-four ORFs were identified on the phiPV83-pro genome, including two extra operons, lukM-lukF-PV and orfs63-64. The lukM-lukF-PV cluster was located 2.1 kb upstream of the attL site. The most striking feature of the phiPV83-pro genome was a constituent of at least 4 regions from phi11, phiPVL, and other phages, i.e., (i) att sites identical with those of phi11, (ii) a cos sequence and the genes encoding packaging and head proteins of phiPVL (occupied half region of phiPV83-pro), and (iii) the other two regions which showed no significant similarity with known phages (occupied about 40% of phiPV83-pro). Furthermore, two insertion sequences, ISSA1 and ISSA2 were integrated into attL site and orf44, respectively. PhiPV83-pro was not induced as phage particles from S. aureus P83 regardless of its treatment with mitomycin C. The insertion of ISSA1 into the attL site was one of the reasons of the failure of the induction of the phage particles by mitomycin C treatment of the strain P83." @default.
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- W2000705460 date "2000-01-01" @default.
- W2000705460 modified "2023-09-24" @default.
- W2000705460 title "Prophage, φPV83-pro, Carrying Panton-Valentine Leukocidin Genes, on the<i>Staphylococcus aureus</i>P83 Chromosome: Comparative Analysis of the Genome Structures of φPV83-pro, φPVL, φ11, and Other Phages" @default.
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- W2000705460 doi "https://doi.org/10.1271/bbb.64.2631" @default.
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