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- W2000709620 abstract "Introduction: Acute necrotising pancreatitis is characterised by an overwhelming inflammatory immune response syndrome (SIRS). This could be followed by a compensatory anti-inflammatory response syndrome (CARS) which may facilitate pancreatic infection. Since the role, if any, of T cells in acute pancreatitis has not been defined, we have determined the expression of the inhibitory T-cell surface molecule CTLA-4 (CD152) in murine models of acute pancreatitis. Methods: Pancreatitis was induced in C57BL6 mice either by 7 hourly injections of caerulein (50 μg/h, i.p.) or by retrograde taurocholate (TC) injections into the pancreatic duct (2%, 3% and 4%). All animals were sacrificed after 12 hours. Serum was evaluated for amylase levels and cytokine secretion (TNFα, IFNγ, IL-6, IL-10, IL-12 and MCP-1). Flow cytometry was employed to evaluate T-cell activation. Granulocyte infiltration was detected by MPO activity measurements and GR-1 staining. Apoptosis was detected by direct 3-OH-nick-end-labelling in the thymus. Splenic CTLA-4 expression was either determined by FACS analysis or antibody labelling of cryosections. Results: TC concentrations of 4% resulted in an overall mortality rate of 85% (50% for 3% TC, 0% for 2% TC), while all caerulein treated animals survived. Pro-inflammatory cytokines, as well as granulocyte activation and tissue infiltration were significantly elevated in taurocholate pancreatitis compared to the caerulein model. Thymus cell apoptosis was found to be significantly increased in taurocholate pancreatitis. Furthermore, we observed a linear correlation of increased CTLA-4 expression with mortality. Conclusion: We show that, also in mice, retrograde duct injection with taurocholate is a feasible and gradable model of experimental pancreatitis. When compared to a milder model (caerulein) taurocholate-induced pancreatitis involves T-cell activation and a possible shift from SIRS to CARS as indicated by increased expression of the inhibitory surface molecule CTLA-4. Further studies will need to clarify whether CTLA-4 could serve as a new marker for pancreatitis severity." @default.
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- W2000709620 date "2005-11-01" @default.
- W2000709620 modified "2023-09-26" @default.
- W2000709620 title "T-CELL RESPONSE IN TAUROCHOLATE PANCREATITIS IN MICE: CTLA-4 A NEW SEVERITY MARKER FOR ACUTE NECROTISING PANCREATITIS?" @default.
- W2000709620 doi "https://doi.org/10.1097/01.mpa.0000193714.83975.7d" @default.
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