Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000716056> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2000716056 endingPage "e52995" @default.
- W2000716056 startingPage "e52995" @default.
- W2000716056 abstract "Endogenous S-nitrosothiols, including S-nitrosoglutathione (GSNO), mediate nitric oxide (NO)-based signaling, inflammatory responses, and smooth muscle function. Reduced GSNO levels have been implicated in several respiratory diseases, and inhibition of GSNO reductase, (GSNOR) the primary enzyme that metabolizes GSNO, represents a novel approach to treating inflammatory lung diseases. Recently, an association between decreased GSNOR expression and human lung cancer risk was proposed in part based on immunohistochemical staining using a polyclonal GSNOR antibody. GSNOR is an isozyme of the alcohol dehydrogenase (ADH) family, and we demonstrate that the antibody used in those studies cross reacts substantially with other ADH proteins and may not be an appropriate reagent. We evaluated human lung cancer tissue arrays using monoclonal antibodies highly specific for human GSNOR with minimal cross reactivity to other ADH proteins. We verified the presence of GSNOR in ≥85% of specimens examined, and extensive analysis of these samples demonstrated no difference in GSNOR protein expression between cancerous and normal lung tissues. Additionally, GSNOR and other ADH mRNA levels were evaluated quantitatively in lung cancer cDNA arrays by qPCR. Consistent with our immunohistochemical findings, GSNOR mRNA levels were not changed in lung cancer tissues, however the expression levels of other ADH genes were decreased. ADH IB mRNA levels were reduced (>10-fold) in 65% of the lung cancer cDNA specimens. We conclude that the previously reported results showed an incorrect association of GSNOR and human lung cancer risk, and a decrease in ADH IB, rather than GSNOR, correlates with human lung cancer." @default.
- W2000716056 created "2016-06-24" @default.
- W2000716056 creator A5001144272 @default.
- W2000716056 creator A5038275537 @default.
- W2000716056 creator A5039363881 @default.
- W2000716056 creator A5043922825 @default.
- W2000716056 creator A5056358604 @default.
- W2000716056 creator A5065548608 @default.
- W2000716056 creator A5078040505 @default.
- W2000716056 date "2012-12-28" @default.
- W2000716056 modified "2023-09-27" @default.
- W2000716056 title "ADH IB Expression, but Not ADH III, Is Decreased in Human Lung Cancer" @default.
- W2000716056 cites W1979611175 @default.
- W2000716056 cites W1990698428 @default.
- W2000716056 cites W1999819498 @default.
- W2000716056 cites W2006819859 @default.
- W2000716056 cites W2018818498 @default.
- W2000716056 cites W2025996212 @default.
- W2000716056 cites W2026585864 @default.
- W2000716056 cites W2027735069 @default.
- W2000716056 cites W2031116994 @default.
- W2000716056 cites W2031137690 @default.
- W2000716056 cites W2070850575 @default.
- W2000716056 cites W2114674175 @default.
- W2000716056 cites W2127297965 @default.
- W2000716056 cites W2164877517 @default.
- W2000716056 cites W2172261903 @default.
- W2000716056 cites W2320525009 @default.
- W2000716056 doi "https://doi.org/10.1371/journal.pone.0052995" @default.
- W2000716056 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3532114" @default.
- W2000716056 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23285246" @default.
- W2000716056 hasPublicationYear "2012" @default.
- W2000716056 type Work @default.
- W2000716056 sameAs 2000716056 @default.
- W2000716056 citedByCount "9" @default.
- W2000716056 countsByYear W20007160562015 @default.
- W2000716056 countsByYear W20007160562016 @default.
- W2000716056 countsByYear W20007160562017 @default.
- W2000716056 countsByYear W20007160562018 @default.
- W2000716056 countsByYear W20007160562020 @default.
- W2000716056 countsByYear W20007160562021 @default.
- W2000716056 countsByYear W20007160562022 @default.
- W2000716056 countsByYear W20007160562023 @default.
- W2000716056 crossrefType "journal-article" @default.
- W2000716056 hasAuthorship W2000716056A5001144272 @default.
- W2000716056 hasAuthorship W2000716056A5038275537 @default.
- W2000716056 hasAuthorship W2000716056A5039363881 @default.
- W2000716056 hasAuthorship W2000716056A5043922825 @default.
- W2000716056 hasAuthorship W2000716056A5056358604 @default.
- W2000716056 hasAuthorship W2000716056A5065548608 @default.
- W2000716056 hasAuthorship W2000716056A5078040505 @default.
- W2000716056 hasBestOaLocation W20007160561 @default.
- W2000716056 hasConcept C121608353 @default.
- W2000716056 hasConcept C142724271 @default.
- W2000716056 hasConcept C153911025 @default.
- W2000716056 hasConcept C203014093 @default.
- W2000716056 hasConcept C204232928 @default.
- W2000716056 hasConcept C2776256026 @default.
- W2000716056 hasConcept C502942594 @default.
- W2000716056 hasConcept C54355233 @default.
- W2000716056 hasConcept C71924100 @default.
- W2000716056 hasConcept C86803240 @default.
- W2000716056 hasConceptScore W2000716056C121608353 @default.
- W2000716056 hasConceptScore W2000716056C142724271 @default.
- W2000716056 hasConceptScore W2000716056C153911025 @default.
- W2000716056 hasConceptScore W2000716056C203014093 @default.
- W2000716056 hasConceptScore W2000716056C204232928 @default.
- W2000716056 hasConceptScore W2000716056C2776256026 @default.
- W2000716056 hasConceptScore W2000716056C502942594 @default.
- W2000716056 hasConceptScore W2000716056C54355233 @default.
- W2000716056 hasConceptScore W2000716056C71924100 @default.
- W2000716056 hasConceptScore W2000716056C86803240 @default.
- W2000716056 hasIssue "12" @default.
- W2000716056 hasLocation W20007160561 @default.
- W2000716056 hasLocation W20007160562 @default.
- W2000716056 hasLocation W20007160563 @default.
- W2000716056 hasLocation W20007160564 @default.
- W2000716056 hasLocation W20007160565 @default.
- W2000716056 hasOpenAccess W2000716056 @default.
- W2000716056 hasPrimaryLocation W20007160561 @default.
- W2000716056 hasRelatedWork W1515918626 @default.
- W2000716056 hasRelatedWork W2079747906 @default.
- W2000716056 hasRelatedWork W2351536507 @default.
- W2000716056 hasRelatedWork W2356586383 @default.
- W2000716056 hasRelatedWork W2360741474 @default.
- W2000716056 hasRelatedWork W2361688476 @default.
- W2000716056 hasRelatedWork W2391396826 @default.
- W2000716056 hasRelatedWork W2392778271 @default.
- W2000716056 hasRelatedWork W4253419875 @default.
- W2000716056 hasRelatedWork W4288213499 @default.
- W2000716056 hasVolume "7" @default.
- W2000716056 isParatext "false" @default.
- W2000716056 isRetracted "false" @default.
- W2000716056 magId "2000716056" @default.
- W2000716056 workType "article" @default.