Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000716978> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2000716978 abstract "Fish allergens can trigger life-threatening reactions in predisposed individuals, which is especially important in countries where seafood is an integrate part of the diet. The major fish allergen parvalbumins are abundant in the white muscle of many fish species. Parvalbumin belongs to the family of EF-hand proteins and has a globular shape containing six helical parts and two active Ca2+ binding sites.High pressure is known to unfold proteins. We performed high pressure FTIR experiments, to explore the T-p phase diagram of cod parvalbumin. This method allows following the secondary structure of the protein as well as the Ca2+ binding to the aspartic glutamic acid residues.The infrared spectrum of parvalbumin is characteristic for the helical conformation, in agreement with the crystal structure, but without adding Ca2+ we found that the sample does not adopt the fully ordered structure. A marked transition in the structure of the parvalbumin was observed with the central point of 0.5 GPa (at room temperature). We assign this change to a native-molten globule transition on the basis of the position of the amide I band of the infrared spectrum. This transition was reversible, which also supports the lack of complete unfolding.Temperature experiments show a two-step unfolding of the protein. Above 45°C we have molten globule kind of loosened structure, and the complete unfolding occurs above 70°C. The unfolding is accompanied with aggregation of the protein. The aggregation can however be prevented by relatively low pressure of 0.2 GPa.Using the above phase transition points the T-p phase diagram of parvalbumin was determined in presence of Ca2+. It shows a very complex pattern, containing two different molten globule phases besides the native, unfolded and aggregated ones." @default.
- W2000716978 created "2016-06-24" @default.
- W2000716978 creator A5038035476 @default.
- W2000716978 creator A5048474775 @default.
- W2000716978 creator A5064087581 @default.
- W2000716978 creator A5074952501 @default.
- W2000716978 date "2012-01-01" @default.
- W2000716978 modified "2023-10-07" @default.
- W2000716978 title "Temperature-Pressure Stability and T-P Phase Diagram of Parvalbumin" @default.
- W2000716978 doi "https://doi.org/10.1016/j.bpj.2011.11.326" @default.
- W2000716978 hasPublicationYear "2012" @default.
- W2000716978 type Work @default.
- W2000716978 sameAs 2000716978 @default.
- W2000716978 citedByCount "0" @default.
- W2000716978 crossrefType "journal-article" @default.
- W2000716978 hasAuthorship W2000716978A5038035476 @default.
- W2000716978 hasAuthorship W2000716978A5048474775 @default.
- W2000716978 hasAuthorship W2000716978A5064087581 @default.
- W2000716978 hasAuthorship W2000716978A5074952501 @default.
- W2000716978 hasBestOaLocation W20007169781 @default.
- W2000716978 hasConcept C105168689 @default.
- W2000716978 hasConcept C118493188 @default.
- W2000716978 hasConcept C121332964 @default.
- W2000716978 hasConcept C133571119 @default.
- W2000716978 hasConcept C141547260 @default.
- W2000716978 hasConcept C149288129 @default.
- W2000716978 hasConcept C169760540 @default.
- W2000716978 hasConcept C178790620 @default.
- W2000716978 hasConcept C185592680 @default.
- W2000716978 hasConcept C2778439535 @default.
- W2000716978 hasConcept C44280652 @default.
- W2000716978 hasConcept C55493867 @default.
- W2000716978 hasConcept C62614982 @default.
- W2000716978 hasConcept C8010536 @default.
- W2000716978 hasConcept C85906118 @default.
- W2000716978 hasConcept C86803240 @default.
- W2000716978 hasConcept C97355855 @default.
- W2000716978 hasConceptScore W2000716978C105168689 @default.
- W2000716978 hasConceptScore W2000716978C118493188 @default.
- W2000716978 hasConceptScore W2000716978C121332964 @default.
- W2000716978 hasConceptScore W2000716978C133571119 @default.
- W2000716978 hasConceptScore W2000716978C141547260 @default.
- W2000716978 hasConceptScore W2000716978C149288129 @default.
- W2000716978 hasConceptScore W2000716978C169760540 @default.
- W2000716978 hasConceptScore W2000716978C178790620 @default.
- W2000716978 hasConceptScore W2000716978C185592680 @default.
- W2000716978 hasConceptScore W2000716978C2778439535 @default.
- W2000716978 hasConceptScore W2000716978C44280652 @default.
- W2000716978 hasConceptScore W2000716978C55493867 @default.
- W2000716978 hasConceptScore W2000716978C62614982 @default.
- W2000716978 hasConceptScore W2000716978C8010536 @default.
- W2000716978 hasConceptScore W2000716978C85906118 @default.
- W2000716978 hasConceptScore W2000716978C86803240 @default.
- W2000716978 hasConceptScore W2000716978C97355855 @default.
- W2000716978 hasLocation W20007169781 @default.
- W2000716978 hasOpenAccess W2000716978 @default.
- W2000716978 hasPrimaryLocation W20007169781 @default.
- W2000716978 hasRelatedWork W114432466 @default.
- W2000716978 hasRelatedWork W1218984382 @default.
- W2000716978 hasRelatedWork W1515480883 @default.
- W2000716978 hasRelatedWork W1964038158 @default.
- W2000716978 hasRelatedWork W1973802485 @default.
- W2000716978 hasRelatedWork W1977630287 @default.
- W2000716978 hasRelatedWork W1994211676 @default.
- W2000716978 hasRelatedWork W1996830377 @default.
- W2000716978 hasRelatedWork W1996910717 @default.
- W2000716978 hasRelatedWork W2018513205 @default.
- W2000716978 hasRelatedWork W2030238973 @default.
- W2000716978 hasRelatedWork W2049003834 @default.
- W2000716978 hasRelatedWork W2058629823 @default.
- W2000716978 hasRelatedWork W2058641297 @default.
- W2000716978 hasRelatedWork W2071573902 @default.
- W2000716978 hasRelatedWork W2073104483 @default.
- W2000716978 hasRelatedWork W2077257989 @default.
- W2000716978 hasRelatedWork W2093047483 @default.
- W2000716978 hasRelatedWork W2171810203 @default.
- W2000716978 hasRelatedWork W2433404105 @default.
- W2000716978 isParatext "false" @default.
- W2000716978 isRetracted "false" @default.
- W2000716978 magId "2000716978" @default.
- W2000716978 workType "article" @default.