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- W2000718549 abstract "Somatostatin (SS) was produced in Escherichia coli (E. coli) as a 110 aminoacid fused protein, C-L-Met-SS, consisting of somatomedin C (Phe23Glu46) [1], human interferon-γ (Cys1-Leu59) [1], methionine and 14 aminoacids of SS. High-level expression of the fused protein, C-L-Met-SS, was achieved with pCLSStrpN2, a plasmid containing trp promoter. The fused protein, C-L-Met-SS, which was formed as a insoluble inclusion body in E. coli, was easily cleaved with cyanogen bromide to give reduced SS (SS(R)). The SS(R) was purified efficiently by gelfiltration chromatography, refolded under dilution, and further purified by reversed phase HPLC to give biologically active SS. The yield was 6.6 mg of the purified SS/20 L culture of E. Coli." @default.
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- W2000718549 date "1989-03-01" @default.
- W2000718549 modified "2023-09-26" @default.
- W2000718549 title "High-Level Expression and Efficient Purification of Recombinant Somatostatin inEscherichia Coli" @default.
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- W2000718549 doi "https://doi.org/10.1002/btpr.5420050110" @default.
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