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- W2000720593 abstract "A simple and rapid one-step method for establishing azaguanine resistant (Agr) hybridomas, which can be used as a fusion partner for the construction of triomas (hybridoma x splenocyte), has been developed. The method relies on cloning the hybridoma cells in soft agar supplemented with 20 μg/ml 8-azaguanine. The drug-resistant subclones were isolated after 3–5 days, in comparison with 4–5 weeks reported for the convetional adaptation method. The high frequency (about 10−3) of Agr-mutants achieved by the cloning method was demonstrated with five different hybridoma clones. One of the derived Agr-hybridomas was fused with mouse immune spleen cells in order to demonstrate its suitability for the generation of triomas secreting bispecific monoclonal antibodies." @default.
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- W2000720593 date "1991-12-01" @default.
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- W2000720593 title "One-step method for establishing 8-azaguanine-resistant hybridomas suitable for the preparation of triomas" @default.
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- W2000720593 doi "https://doi.org/10.1016/0022-1759(91)90333-b" @default.
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