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- W2000720840 abstract "Background The possibility that the hyperstimulatory effect of gonadotropins may be important etiologically in the genesis of some cases of ovarian cancer is still under discussion. Few flow and image cytometric studies report on DNA aneuploid granulosa cells in follicular fluid from women undergoing in vitro fertilization (IVF). The reports are very controversial. The aim of the study was to analyse relationship between ultrasound-determined follicular size and DNA ploidy of granulosa cells from gonadotropin-stimulated follicles. Method The granulosa-luteal cells were obtained from women undergoing an IVF-ET program at the University Department of obstetrics and Gynecology Ljubljana. Seventy aspirates of individual follicles (transvaginal determined size, range 15 mm – 30 mm in diameter) from gonadotropin-stimulated cycles were analysed. Cytospins were prepared and fixed in delaunay fixative. All the slides were stained by the feulgen thionin method. Image DNA analyses was performed on automated high-resolution image cytometer. The DNA index was calculated to define DNA ploidy. Results All granulosa cell samples were found to be diploid. Conclusion Regardless of different ultrasound-determined size of follicles, all analysed granulosa cells have diploid DNA content. Acknowledgements Supported by grants from ministry of Research and technology republic of Slovenia (No. j3-0428–032–98)." @default.
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- W2000720840 date "2000-10-01" @default.
- W2000720840 modified "2023-09-27" @default.
- W2000720840 title "WS17-03Relationship between ultrasound-determined follicular size and DNA ploidy of human granulosa cells" @default.
- W2000720840 doi "https://doi.org/10.1046/j.1469-0705.2000.00009-1-111.x" @default.
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