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• W2000726584 abstract "The association constants for the interaction of BALB/c (UPC 10, Y5476, W3082, and UPC 61) and NZB (PC 3660) myeloma anti-D-fructans in pure form, or in ascitic fluids, with high-molecular-weight levans (Ka) and with such low-molecular-weight compounds as rye-grass levan, inulin, sucrose, and D-fructo-oligosaccharides were determined by affinity electrophoresis, measuring the extent of retardation of the D-fructan-specific band by levan and its restoration by the low-molecular-weight compounds and oligosaccharide haptens. With different levans, Ka values ranged from 1.14 x 105 to 1.52 x 106 mL/g for PC 3660, 1.35 x 105 to 6.45 x 105 mL/g for UPC 10, 1.0 x 104 to 7.9 x 104 mL/g for Y5476, 7.63 x 103 to 6.38 x 104 mL/g for W3082, and 3.35 x 103 to 1.54 x 104 mL/g for UPC 61. The retarded, D-fructan-specific bands of W3082 and UPC 61 were restored by inulin, having β-D-(2→1)-linkages, and rye-grass levan, having β-D-(2→6)-linkages, and those of PC 3660, UPC 10, and Y5476 by rye-grass levan. The values of inulin with W3082 and UPC 61 were 10 times those of rye-grass levan. The values of inulin (3.65 x 105M-1 for W3082, and 4.44 x l05M-1 for UPC 61) were very similar to those of [β-D-Fruf-(2→1)]2-β-D-Fruf-(2→6)-D-Glc (3) (3.95 x 105M-1 for W3082, and 4.5 x 105M-1 for UPC 61). With sucrose and the D-fructo-oligosaccharides, the order of values of W3082 and UPC 61 was 3 > β-D-Fruf-(2→1)-β-D-Fruf-(2→6)- D-Glc > sucrose > β-D-Fruf-(2→6)-D-Glc. With rye-grass levan, values were 4.25 x 105M-1 for PC 3660, 2.7 x 105M-1 for UPC 10, and 8.77 x 104M-1 for Y5476. These results confirm earlier findings that W3082 and UPC 61 have dual specificity for β-(2→1) and β(2→6) D-fructofuranosyl linkages, that PC 3660, Y 5476, and UPC 10 have specificity for β-(2→6) D-fructofuranosyl linkages, and that the combining sites of W3082 and UPC 61 are most complementary to the tetrasaccharide 3. That W3082 and Y5476 share the same, cross-reacting idiotype, although their combining sites differ in specificity, provides further evidence that these two properties do not run parallel." @default.
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• W2000726584 title "Binding constants of levans and D-fructo-oligosaccharides to BALB/c and NZB D-fructan-specific, myeloma proteins, determined by affinity electrophoresis" @default.
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• W2000726584 doi "https://doi.org/10.1016/s0008-6215(00)85525-1" @default.
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