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• W2000729213 abstract "Spacing mutations have been constructed between an SV40 72 bp enhancer element and the Adenovirus-2 major late promoter upstream element (Ad2MLP-UE), the Ad2MLP-UE and the Ad2MLP TATA-box and the SV40 72 bp enhancer and the TATA-box in the absence of the Ad2MLP-UE. A clear periodic pattern of transcription from the Ad2MLP was obtained by inserting multiples of half of a DNA turn between the SV40 enhancer and the Ad2MLP-UE. We interpret this as a stereospecific requirement for the spatial alignment between the enhancer element and either the Ad2MLP-UE or the TATA-box. Insertions between the upstream element and the TATA-box did not give a periodic transcription pattern, though insertion of half of a DNA turn increased the steady-state level of specific RNA. Unexpectedly, spacing mutations between the enhancer and TATA-box in which the upstream element had been point mutated gave indications of stereospecificity, whereas the same insertions with a deleted upstream element showed little requirement for a precise stereoalignment." @default.
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• W2000729213 date "1989-01-01" @default.
• W2000729213 modified "2023-10-18" @default.
• W2000729213 title "Stereospecific relationships between elements in an SV40/Adenovirus-2 heterologous promoter" @default.
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• W2000729213 doi "https://doi.org/10.1093/nar/17.17.6903" @default.
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