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- W2000731948 abstract "RNA secondary structure prediction using free energy minimization is one method to gain an approximation of structure. Constraints generated by enzymatic mapping or chemical modification can improve the accuracy of secondary structure prediction. We report a facile method that identifies single-stranded regions in RNA using short, randomized DNA oligonucleotides and RNase H cleavage. These regions are then used as constraints in secondary structure prediction. This method was used to improve the secondary structure prediction of Escherichia coli 5S rRNA. The lowest free energy structure without constraints has only 27% of the base pairs present in the phylogenetic structure. The addition of constraints from RNase H cleavage improves the prediction to 100% of base pairs. The same method was used to generate secondary structure constraints for yeast tRNAPhe, which is accurately predicted in the absence of constraints (95%). Although RNase H mapping does not improve secondary structure prediction, it does eliminate all other suboptimal structures predicted within 10% of the lowest free energy structure. The method is advantageous over other single-stranded nucleases since RNase H is functional in physiological conditions. Moreover, it can be used for any RNA to identify accessible binding sites for oligonucleotides or small molecules." @default.
- W2000731948 created "2016-06-24" @default.
- W2000731948 creator A5042677350 @default.
- W2000731948 creator A5068295788 @default.
- W2000731948 creator A5068310777 @default.
- W2000731948 creator A5070993847 @default.
- W2000731948 date "2009-07-13" @default.
- W2000731948 modified "2023-10-14" @default.
- W2000731948 title "Improvement of RNA secondary structure prediction using RNase H cleavage and randomized oligonucleotides" @default.
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- W2000731948 doi "https://doi.org/10.1093/nar/gkp587" @default.
- W2000731948 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2764423" @default.
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